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LC-MS/MS method for rapidly and quantitatively detecting four cholic acid components in blood plasma

A technology of quantitative detection and detection method, which is applied in the direction of measurement device, material separation, analysis of materials, etc., can solve the problems of inability to achieve accurate quantitative detection, low free concentration, and low detection concentration.

Pending Publication Date: 2020-10-27
成都华西海圻医药科技有限公司
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Problems solved by technology

The current common detection method is to detect binding UDCA and CDCA by HPLC, and for free UDCA and CDCA, due to the low detection concentration, conventional HPLC or LCMS methods cannot be accurately quantified, and at the same time, taurine in bile acids Ursodeoxycholic acid (TUDCA) and taurochenodeoxycholic acid (TCDCA) also have low free concentrations in the body, and accurate quantitative detection cannot be achieved at present

Method used

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  • LC-MS/MS method for rapidly and quantitatively detecting four cholic acid components in blood plasma
  • LC-MS/MS method for rapidly and quantitatively detecting four cholic acid components in blood plasma
  • LC-MS/MS method for rapidly and quantitatively detecting four cholic acid components in blood plasma

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Embodiment 1

[0035] Example 1. Detection of Ursodeoxycholic Acid, Chenodeoxycholic Acid, Tauroursodeoxycholic Acid and Taurochenodeoxycholic Acid in Plasma

[0036] (1) Establishment of a standard curve for bile acids

[0037] a. Preparation of reference substance solution: Weigh 10 mg ursodeoxycholic acid, chenodeoxycholic acid, tauroursodeoxycholic acid sodium and taurochenodeoxycholic acid standard reference substance respectively, and ursodeoxycholic acid , chenodeoxycholic acid were transferred to a 5mL brown volumetric flask, add methanol solution to shake and dissolve, and then make the volume to the mark; , add methanol solution to shake and dissolve, and then set the volume to the mark to obtain the reference substance solution;

[0038] b. Preparation of series concentration standard working solution: take step a reference substance solution, add 50% methanol aqueous solution to prepare series concentration reference substance solution, wherein, the concentration of ursodeoxycho...

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Abstract

The invention discloses an LC-MS / MS method for rapidly and quantitatively detecting four cholic acid components in blood plasma. The method comprises the following steps of: (1) establishing a cholicacid component standard curve: a, preparing a reference substance solution, b, preparing a series of concentration matrix mixed reference substance solutions, and c, respectively sucking the series ofconcentration matrix mixed reference substance solutions, and injecting the solutions into an LC-MS / MS instrument, and determining the peak area to obtain the cholic acid component standard curve; (2) determining the content of cholic acid components in a to-be-determined sample: d, preparing a test solution, and e, determining the test solution. According to the LC-MS / MS method for rapidly detecting the four cholic acid components in the blood plasma, free ursodeoxycholic acid, chenodeoxycholic acid, tauroursodeoxycholic acid and / or taurochenodeoxycholic acid in the blood can be detected atthe same time, the result is accurate, the automation degree is high, the speed is high, the sensitivity is high, and the method has very important significance.

Description

technical field [0001] The invention relates to quantitative drug concentration analysis in vivo, in particular to an LC-MS / MS method for rapidly quantitatively detecting four kinds of cholic acid components in blood plasma. Background technique [0002] Animal bile is a kind of traditional Chinese medicine, which has important physiological significance and clinical application value. The medicine with bear bile as the main component is mainly used to treat liver and gallbladder diseases, among which ursodeoxycholic acid (UDCA) and chenodeoxycholic acid ( CDCA) is an endogenous bile acid, and UDCA and CDCA mainly exist in the form of taurine in bear bile powder. The current common detection method is to detect binding UDCA and CDCA by HPLC, and for free UDCA and CDCA, due to the low detection concentration, conventional HPLC or LCMS methods cannot be accurately quantified, and at the same time, taurine in bile acids Ursodeoxycholic acid (TUDCA) and taurochenodeoxycholic ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N2030/324
Inventor 赖力胡双龚梅
Owner 成都华西海圻医药科技有限公司
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