Method for detecting concentration of anti-dementia drugs in serum

Abstract

The invention provides a method for detecting concentration of anti-dementia drugs in serum. The method has the advantages that the pretreatment operation is simple and rapid, the use amount of samples is small, the analysis time is short, and separation and detection of the three anti-dementia drugs in the serum can be completed within 4.5 min; matrix interference can be greatly eliminated by adopting an isotope internal standard method for quantification, the influence of conditions such as a pretreatment process, a sample loading volume and a flowing phase is avoided, and accurate quantification can be achieved; meanwhile, the clinical high-throughput sample detection requirements can be met, and a simple and rapid detection method is provided for concentration monitoring of clinical anti-dementia drugs.

Description

technical field [0001] The invention belongs to the technical field of blood detection, specifically a method for detecting the concentration of anti-dementia drugs in serum, wherein the anti-dementia drugs are respectively: Memantine (MMT), rivastigmine (Rivastigmine, RVT) and donepezil (Donepezil, DNP). Background technique [0002] Therapeutic drug monitoring (Therapeutic drug monitoring, TDM) is a method for tailoring the dosage regimen for different individual patients. Dosage titration of individual patients is performed by quantitatively measuring serum or drug concentration in serum, so as to obtain the best curative effect, better tolerance, and reduce toxic and side effects. [0003] The traditional microbial method is time-consuming and cumbersome; while the immunoassay method is expensive, has poor specificity, and is difficult to achieve high-throughput detection. The key problem is that due to the existence of metabolites, antibodies may cross-react and the me...

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Problems solved by technology

[0007] The antidementia drugs involved above have been reported in the literature to measure the drug content in human or animal serum by using HPLC-UV, GC-MS and LC-MS / MS methods. The literature "Simultaneous determination of antidementiadrugs in human plasma: Procedure transfer from HPLC–MS to UPLC–MS / MS” reported a method for the detection of anti-dementia drugs in plasma by liquid mass spectrometry. The pretreatment is complicated, nitrogen blowing is required, and the sample requires a large amount of 250 μL for each sample. Other reports mostly use The liquid-liquid extraction method may require derivatization of serum samples, which has disadvantages such as cumbersome operation steps, large amount of serum, long analysis time for a single sample, and low sensitivity.

For another example, Chinese Patent No. (CN 110361483A) discloses "a kit for monitoring donepezil drug concentration in blood and its detection method", which uses multi-dimensional on-line solid-phase extraction liquid chromatography to only measure donepezil drug in blood. , although the operation is simple, but the sample volume is large (requires 4mL), the sensitivity is low (quantitative limit 10ng / mL), and the analysis time is as long as 17 minutes

[0008] In summary, the existing methods have long detection time, single detection items, and low efficiency, and basically use external standard method or chemical substances similar to the analyte as internal standard for quantitative analysis, which is greatly affected by matrix effect , the lack of accuracy

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