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A serum-free medium for mesenchymal stem cells without pre-coating and its application

A serum-free culture medium and stromal stem cell technology, applied in the field of mesenchymal stem cell serum-free culture medium, can solve problems such as being unfavorable for large-scale industrial production, and achieve a state that is beneficial to large-scale industrial production, cost saving, and cell growth. good effect

Active Publication Date: 2021-07-30
BIOISLAND LAB +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For serum-free medium, the existing products (including the above-cited patents CN108300690A and CN108251359A) need to pre-coat the culture container, because without pre-coating, the cell adherence is significantly lower than that with serum culture medium, but the pre-coating operation is not conducive to large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] This embodiment provides a serum-free medium for mesenchymal stem cells that does not require pre-coating, and its formula is shown in the following table:

[0043] Element concentration recombinant human serum albumin 2μg / mL Recombinant Human Basic Fibroblast Growth Factor (bFGF) 5ng / L Transforming growth factor beta 1 (TGF-beta1) 5ng / L DGEA peptide 20μg / mL Ascorbyl 2-phosphate 150μM penicillin / streptomycin 100U / mL / 100U / mL Dexamethasone 75nM Lipids 40pg / mL ITS 1% α-MEM medium /

[0044] Its preparation method is: in 500 mL α-MEM culture medium, add the recombinant human serum albumin, recombinant human basic fibroblast growth factor, transforming growth factor β1, DGEA peptide, ascorbic acid 2-phosphate, Penicillin / streptomycin solution, dexamethasone, and lipids.

Embodiment 2

[0046] This embodiment provides a serum-free medium for mesenchymal stem cells that does not require pre-coating, and its formula is shown in the following table:

[0047] Element concentration recombinant human serum albumin 3μg / mL Recombinant Human Basic Fibroblast Growth Factor (bFGF) 7.5ng / L Transforming growth factor beta 1 (TGF-beta1) 7.5ng / L DGEA peptide 20μg / mL Ascorbyl 2-phosphate 180μM penicillin / streptomycin 100U / mL / 100g / mL Dexamethasone 100nM Lipids 30pg / mL ITS 1% α-MEM medium /

[0048] Its preparation method refers to embodiment 1.

Embodiment 3

[0050] This embodiment provides a serum-free medium for mesenchymal stem cells that does not require pre-coating, and its formula is shown in the following table:

[0051] Element concentration recombinant human serum albumin 7μg / mL Recombinant Human Basic Fibroblast Growth Factor (bFGF) 7.5ng / L Transforming growth factor beta 1 (TGF-beta1) 7.5ng / L DGEA peptide 20μg / mL Ascorbyl 2-phosphate 120μM penicillin / streptomycin 100U / mL / 100g / mL Dexamethasone 75nM Lipids 40pg / mL ITS 1% α-MEM medium /

[0052] Its preparation method refers to embodiment 1.

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Abstract

The invention relates to a serum-free medium for mesenchymal stem cells without pre-coating and an application thereof. The serum-free medium for mesenchymal stem cells without pre-coating includes basal medium and additives, and the additives include recombinant human Serum albumin, recombinant human basic fibroblast growth factor, transforming growth factor beta 1 and DGEA peptide. The medium is a completely xeno-free culture system, so it will not cause heterogeneous contamination; creatively add DGEA peptide, and cooperate with recombinant human serum albumin and recombinant human basic fibroblasts to grow Factors, transforming growth factor β1 and other additives make it possible to culture mesenchymal stem cells directly without pre-coating the culture container. good. This greatly simplifies the operation steps, saves costs, and is conducive to large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and relates to a serum-free medium for mesenchymal stem cells and an application thereof, in particular to a serum-free medium for mesenchymal stem cells without pre-coating and an application thereof. Background technique [0002] Mesenchymal Stem Cells (MSCs) are pluripotent cells that can differentiate into a variety of cell types, including: osteoblasts, chondrocytes, and adipocytes. This ability to differentiate has been demonstrated in many situations Identified, including specific cells and tissues in living animals and their counterparts grown in tissue culture. In recent years, due to the wide range of therapeutic uses of MSCs and their great potential to repair damaged or diseased tissues, MSCs have become a hot spot in scientific research and clinical research. [0003] The currently used MSC medium basically contains fetal bovine serum (FBS), because it can provide the necessary...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0662C12N5/0668C12N2500/24C12N2500/36C12N2500/38C12N2500/90C12N2501/115C12N2501/15C12N2501/39C12N2501/998C12N2501/999
Inventor 陈东煌陈海佳王小燕崔梓豪姜交华戚康艺
Owner BIOISLAND LAB
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