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Mesenchymal stem cell serum-free medium without pre-coating and application thereof

A technology of serum-free medium and mesenchymal stem cells, which is applied in the field of serum-free medium for mesenchymal stem cells, and can solve problems such as unfavorable large-scale industrial production

Active Publication Date: 2020-10-23
BIOISLAND LAB +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For serum-free medium, existing products (including the above-mentioned cited patents CN108300690A and CN108251359A) all need to pre-coat the culture container, because if not pre-coated, the adhesion of the cells is significantly lower than that containing serum culture medium, but the pre-coating operation is not conducive to large-scale industrial production

Method used

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  • Mesenchymal stem cell serum-free medium without pre-coating and application thereof
  • Mesenchymal stem cell serum-free medium without pre-coating and application thereof
  • Mesenchymal stem cell serum-free medium without pre-coating and application thereof

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Experimental program
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Effect test

Embodiment 1

[0044] This embodiment provides a serum-free medium for mesenchymal stem cells that does not require pre-coating, and its formula is shown in the following table:

[0045]

[0046]

[0047] Its preparation method is: in 500mL α-MEM medium, sequentially add recombinant human serum albumin, recombinant human basic fibroblast growth factor, transforming growth factor β1, DGEA peptide, ascorbic acid 2-phosphate, penicillin / Streptomycin solution, dexamethasone, and lipids.

Embodiment 2

[0049] This embodiment provides a serum-free medium for mesenchymal stem cells that does not require pre-coating, and its formula is shown in the following table:

[0050]

[0051]

[0052] Its preparation method refers to embodiment 1.

Embodiment 3

[0054] This embodiment provides a serum-free medium for mesenchymal stem cells that does not require pre-coating, and its formula is shown in the following table:

[0055]

[0056] Its preparation method refers to embodiment 1.

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Abstract

The invention relates to a mesenchymal stem cell serum-free medium without pre-coating and application thereof. The mesenchymal stem cell serum-free medium without pre-coating comprises a basal culture medium and additives, wherein the additives comprise recombinant human serum albumin, recombinant human basic fibroblast growth factors, transforming growth factors beta1 and DGEA peptide. The serum-free medium is a completely non-heterologous (Xeno-free) culture system, so that heterologous pollution cannot be caused; and the DGEA peptide is creatively added, in cooperation with the recombinanthuman serum albumin, the recombinant human basic fibroblast growth factor, the transforming growth factor beta1 and other additive components, the mesenchymal stem cells are directly cultured withoutpre-coating a culture container when the mesenchymal stem cells are cultured, the cells have high adherence rate, amplification multiple and cell viability, and the cell growth state is good. Therefore, the operation steps are greatly simplified, the cost is saved, and large-scale industrial production is facilitated.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and relates to a serum-free medium for mesenchymal stem cells and an application thereof, in particular to a serum-free medium for mesenchymal stem cells without pre-coating and an application thereof. Background technique [0002] Mesenchymal Stem Cells (MSCs) are pluripotent cells that can differentiate into a variety of cell types, including: osteoblasts, chondrocytes, and adipocytes. This ability to differentiate has been demonstrated in a variety of situations Identified, including specific cells and tissues in living animals and their counterparts grown in tissue culture. In recent years, due to the wide range of therapeutic uses of MSCs and their great potential to repair damaged or diseased tissues, MSCs have become a hot spot in scientific research and clinical research. [0003] The currently used MSC medium basically contains fetal bovine serum (FBS), because it can provide the n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0662C12N5/0668C12N2500/24C12N2500/36C12N2500/38C12N2500/90C12N2501/115C12N2501/15C12N2501/39C12N2501/998C12N2501/999
Inventor 陈东煌陈海佳王小燕崔梓豪姜交华戚康艺
Owner BIOISLAND LAB
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