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Preparation method of feather peptide powder

A feather and peptide powder technology, applied in the field of resource utilization of poultry slaughtering waste, can solve problems such as the difficulty of orthogonal experiments, and achieve the effects of easy control of process parameters, resource saving, and simple operation

Inactive Publication Date: 2020-10-23
ZHEJIANG UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, there is a need for further improvement, but it is difficult to further improve

Method used

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  • Preparation method of feather peptide powder
  • Preparation method of feather peptide powder
  • Preparation method of feather peptide powder

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Experimental program
Comparison scheme
Effect test

Embodiment

[0026] 1. Test materials:

[0027] Chicken feathers; alkaline protease (200U / mg), keratinase (70,000 U / g), sodium hydroxide (analytical pure), hydrochloric acid (analytical pure), BCA total protein concentration determination kit, standard peptide markers, acetonitrile (chromatographic grade).

[0028] 2. Test equipment:

[0029] pH desktop acidity meter, water bath, high-speed centrifuge, high-pressure steam sterilizer, microplate reader, ultra-pure water device, microplate fast oscillator, desktop air constant temperature oscillator, dryer, gel chromatograph.

[0030] 3. Test method and content:

[0031] 1 Feather pretreatment: feather cleaning, impurity removal, shredder crushing, about 1-2 cm long, sterilizing in a high-pressure steam sterilizer (121°C, 20min), dry storage for later use.

[0032] 2 Determination of soluble protein content: determined by BCA kit.

[0033] 3 Single factor test to determine the optimal enzymatic hydrolysis conditions for multiple enzymes ...

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Abstract

The invention relates to the technical field of resource utilization of poultry slaughtering waste, and particularly discloses a preparation method of feather peptide powder. The method takes poultryfeathers as a raw material, and is characterized by comprising the following steps of crushing the feathers, and then putting the crushed feathers into a container; injecting water, adding 50-400 U ofkeratinase and 1,000,000-3,600,000 U of alkaline protease into each 1g of feather, adjusting the pH to be 10-11.5, reacting at 40-50 DEG C for 4h-8h, and drying filtrate to obtain a peptide powder product. The process disclosed by the invention is simple to operate, safe and efficient, and has great significance for recycling slaughtering wastes such as feathers. The content of soluble protein inthe filtrate of the product is higher than 680 ug / ml, the peptide content in peptide powder is higher than 95%, the relative molecular mass of peptide is below 2000 Dalton, and the product has higherpurity, can be processed into an animal protein feed raw material, has wider application range and is suitable for wide popularization and application.

Description

technical field [0001] The invention relates to the technical field of resource utilization of poultry slaughter waste, and mainly relates to a preparation method of feather peptide powder. Background technique [0002] With the rapid development of the poultry industry, feathers, as one of the by-products of poultry processing, have a huge annual output. As of 2019, the global annual feather output exceeds 5 million tons, and China's annual feather output exceeds 1 million tons. Inappropriate Disposal is not only a waste of resources, but also pollutes the environment. The protein content in feathers is as high as more than 80% (mainly keratin), and the content of sulfur-containing amino acids ranks first in all natural feeds, which is an excellent protein raw material. However, because keratin contains a large number of disulfide bonds, which play a cross-linking role in the peptide chain of the protein, it is an extremely stable protein that is not easily digested and ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/34A23J3/04A23J1/10A23K20/147
CPCA23J1/10A23J3/04A23J3/341A23K20/147
Inventor 占秀安王殿纯呼惠娟孟庆丰张开臣王园园徐义斌王江水孔德军邵明晨孔令华邵媛媛马灿峰
Owner ZHEJIANG UNIV
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