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Anti-aging bifidobacterium animalis and application thereof

An animal bifidobacteria, anti-aging technology, applied in the field of bioengineering, can solve problems such as few other diseases, and achieve the effect of increasing peroxidase activity, reducing brain inflammatory factors, and reversing anxiety-like behaviors

Pending Publication Date: 2020-10-16
JIANGXI SHANXING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many kinds of microbial preparations at home and abroad, they are generally used to treat intestinal diseases such as gastroenteritis, constipation, diarrhea, etc., and are rarely used to treat other diseases

Method used

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  • Anti-aging bifidobacterium animalis and application thereof
  • Anti-aging bifidobacterium animalis and application thereof
  • Anti-aging bifidobacterium animalis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Acquisition and Identification of Animal Bifidobacteria

[0031] 1. Acquisition and identification of bifidobacteria

[0032] The feces of centenarians are stored in 50% glycerol, and diluted in sterile phosphate buffered saline (PBS). Select 3-5 appropriate gradients and draw 0.1ml into MRS medium for streak separation. Each dilution The gradient was done in two replicates. Put the petri dish into an incubator at 37°C for anaerobic culture for 48 hours, and then select smooth, convex, neat edges, and white colonies for microscopic examination. The suspected colonies were isolated and purified again in MRS medium according to the above steps until the colonies were completely purified. The purified Bifidobacterium animalis was continuously passaged for 3 times, and then stored at -80°C with a 1:1 mixture of 50% glycerol and bacterial liquid.

[0033] 2. Identification of Animal Bifidobacteria

[0034] After the selected bacterial colonies were continuousl...

Embodiment 2

[0036] Embodiment 2: the acid resistance experiment of animal bifidobacteria

[0037] Take out Bifidobacterium animalis from the ultra-low temperature refrigerator at -80°C and inoculate it in MRS medium, culture it anaerobically overnight at 37°C, then inoculate it into fresh MRS medium at 2%, culture it at 37°C for 24 hours, and dilute it with PBS buffer for 101 , 102, 103, 104, 105 times, centrifuge at 2500rpm for 4min, and discard the supernatant. Add PBS buffer solution with pH of 2, 3, 4, 5, 7, let stand for 4 hours, mix well, take 10uL to smear on the plate, culture in 37°C incubator anaerobically overnight, and count viable bacteria.

[0038] The result is as figure 1 As shown, Bifidobacterium animalis can tolerate PBS incubation with pH=3 and above, and has good acid resistance.

Embodiment 3

[0039] Embodiment 3: bile salt resistance test of animal bifidobacteria

[0040] MRS liquid medium with bile salt concentration of 0%, 0.1%, 0.2%, 0.3% and 0.5% bile salt was prepared in advance. Inoculate the frozen bifidobacteria in MRS medium, culture anaerobically at 37°C overnight, then inoculate them in MRS medium with different concentrations of bile salts at 2% and culture them for 24 hours, dilute them in PBS gradients, shake and mix well, Take 10uL to smear the plate, culture it anaerobically in a 37°C incubator overnight, and count the viable bacteria.

[0041] The result is as figure 2 As shown, Bifidobacterium animalis can survive in various concentrations of bile salt medium, showing good tolerance to bile salt.

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PUM

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Abstract

The invention discloses bifidobacterium animalis 952 with an anti-aging function and an application thereof, and belongs to the technical field of bioengineering. The bacterium is separated from intestinal tracts of the aged, has good acid resistance and cholate resistance, has high DPPH free radical and hydroxyl free radical scavenging capacity, Fe<2+> chelating capacity and lipid peroxidation resistance at the same time, can obviously improve the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), and remarkably reduces the expression level of inflammatory factors inbrain tissue. The bifidobacterium animalis provided by the invention has important practical significance and economic value for application in the aspect of human body aging.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to an anti-aging animal bifidobacterium and its application. Background technique [0002] With the aging population in our country, the incidence of aging-related diseases (Parkinson's disease, Alzheimer's disease, malignant tumors, etc.) continues to rise, which has caused a heavy economic burden to the country and the families of patients. Therefore, finding or developing anti-aging active substances and exploring their mechanisms has become a research hotspot to deal with the problem of population aging. Although many drugs such as metformin, resveratrol, and rapamycin have been proven to have anti-aging effects, they have not been widely promoted due to problems such as high cost, difficult extraction, and relatively large side effects. [0003] More and more studies have shown that probiotics play an important role in delaying human aging. In the process of human agin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23L33/135A61P39/06C12R1/01
CPCA23C9/123A23L33/135A61P39/06A23V2002/00C12R2001/01C12N1/205A23V2400/515A23V2200/302A23V2200/30
Inventor 陈廷涛王京苏邓兆群王盛洲何学松
Owner JIANGXI SHANXING BIOTECHNOLOGY CO LTD
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