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Application of brevibacillus laterosporus MES818 in tomato cultivation

A technology of MES818 and Bacillus brevius, which is applied in the application field of Brevibacillus lateralosporus MES818 in tomato cultivation, can solve the problems of undiscovered tomato blight, damage to the soil ecological environment, and decline in human life quality, so as to promote yield and quality, reduction of occurrence and harm, and low cost

Active Publication Date: 2020-10-09
天津开发区坤禾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of chemical control is good, but the use of chemical pesticides will cause serious pollution of the environment, destroy the soil ecological environment, cause the accumulation of toxic substances, and eventually cause the decline of human life quality
So far, there is no report on the control of tomato blight with Bacillus laterosporus

Method used

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  • Application of brevibacillus laterosporus MES818 in tomato cultivation
  • Application of brevibacillus laterosporus MES818 in tomato cultivation
  • Application of brevibacillus laterosporus MES818 in tomato cultivation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Preparation of the Brevibacillus spp. MES818 bacterial agent for preventing and treating tomato blight

[0044] (1) Shake flask culture: carry out the seed shake flask culture of the bacterial strain activated on the slant, the shake flask medium is beef extract peptone medium, each component and content are: soybean peptone 8-10 grams, beef extract 2-4 grams , 4-5 grams of sodium chloride, 18-20 grams of agar, 1000 milliliters of ultrapure water, and a pH value of 7.0-7.5. Under the conditions of temperature 28°C-32°C and rotation speed of 180-220rpm, shake culture for 18-24h;

[0045] (2) First-level seed culture: the Brevibacillus lateralosporus MES818 strain activated on the test tube slant plane is carried out to Anbei bottle culture, and the Anbei bottle medium is a broth medium, and the medium formula is: 8-10 grams of soybean peptone, 2-4 grams of beef extract, 4-5 grams of sodium chloride, 18-20 grams of agar, 1000 milliliters of ultra-pure water, an...

Embodiment 2

[0051] Example 2 Inhibitory effect of Brevibacillus spp. MES818 on tomato blight

[0052] (1) Test time and place: In May 2019, it will be conducted in the company's R&D laboratory.

[0053] (2) Test method

[0054] (1) Source of tomato blight pathogen: tomato blight was collected from field diseased strains in Wuqing District, Tianjin. After isolation and purification by the company's researchers, it was identified as Rhizoctonia solani. The pathogenicity test showed that Strong pathogenicity.

[0055] (2) Tablet confrontation experiment

[0056] First inoculate tomato solanum blight in the center of the potato agar medium plate, then dip a small amount of activated Brevibacillus spp. MES818 and inoculate it at about 2 cm on both sides of the pathogen, and set the plate only inoculated with tomato solanum blight as blank control. Cultivate at a constant temperature of 28°C. When the blank control is about to cover the entire petri dish, measure the control growth (colony ...

Embodiment 3

[0060] Example 3 Control test of Brevibacillus spp. MES818 on tomato blight

[0061] (1) Test drug

[0062] (1) Microbial agent

[0063] The MES818 liquid formulation prepared in Example 1 was diluted 600 times with water.

[0064] (2) Chemical agents

[0065] 50% thiram wettable powder (Shandong Libang Agrochemical Co., Ltd.), diluted 500 times with water.

[0066] (3) Blank control

[0067] Set only the treatment of spraying clear water as blank control.

[0068] (2) Test time and place

[0069] On June 20, 2019, it was carried out in the nursery room of Tianjin Kunhe Biotechnology Group Co., Ltd.

[0070] (3) Test method

[0071] Sow the tomato seeds in the seedling trays after germination. When the seedlings are about 5 cm high, select seedlings with uniform growth and transplant them into plastic seedling pots with a diameter of 26 cm. One plant per pot, 5 pots per treatment, each Each treatment was set to repeat 3 times. When the seedlings grow about 6-7 true le...

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Abstract

The invention belongs to the technical field of plant cultivation by utilizing microorganisms, and particularly relates to an application of brevibacillus laterosporus MES818 in tomato cultivation. The strain is brevibacillus laterosporus MES818, is preserved in China General Microbiological Culture Collection Center on December 17, 2018, and has a preservation number of CGMCC NO. 16859. The brevibacillus laterosporus MES818 can be applied to prevention and treatment of tomato damping off and tomato yield increase. The brevibacillus laterosporus MES818 disclosed by the invention has the beneficial effects that the brevibacillus laterosporus MES818 has an obvious inhibition effect on tomato damping off pathogenic bacteria Rhizoctonia solani, can reduce the occurrence and harm of tomato damping off, and can promote the improvement of tomato yield and quality.

Description

technical field [0001] The invention belongs to the technical field of cultivating plants by using microorganisms, and in particular relates to the application of Brevibacillus sporogenes MES818 in tomato cultivation. Background technique [0002] Tomatoes are loved by people because of their rich nutrition and good taste. China is the world's largest exporter of tomato products. Tomatoes are of great significance to people's lives and national economic development. However, in recent years, the blight of tomato seedlings has occurred frequently, and the severe disease often causes the death of seedlings in patches. During the seedling cultivation period, the disease is severe in years with continuous rainy weather, less sunlight, and high temperature. Seedlings just unearthed and large seedlings can be affected. The stem base of diseased seedlings turns brown, the diseased part constricts and becomes thinner, and the stems and leaves wither and die; slightly larger seedlin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/60A01N63/22A01P3/00C12R1/07
CPCC12N1/20C05G3/60A01N63/22C12R2001/07C12N1/205
Inventor 顾欣燕肖培英刘刚王秀丽刘金龙鲁玉峰
Owner 天津开发区坤禾生物技术有限公司
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