A kind of priming agent for aerobic fermentation of cow dung and preparation method thereof

An aerobic fermentation and priming agent technology, which is applied in the field of agricultural and rural waste recycling, can solve the problems of shortening the heating time of priming agents, less priming agents, and large differences, so as to improve the quality of aerobic fermentation products, shorten the fermentation cycle, The effect of prolonging the high temperature period

Active Publication Date: 2022-04-26
ACADEMY OF PLANNING & DESIGNING OF THE MINIST OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large differences in livestock and poultry species and feeding patterns, the composition of livestock manure and biogas residues is very complex and varies greatly. There are many researches and developments on priming agents for the aerobic fermentation process of sludge and biogas residues, but based on the aerobic fermentation process of cow dung There are fewer primers developed based on the characteristics of microbial community structure and activity changes, and there are fewer primers that can be used to improve the efficiency of aerobic fermentation of cow dung and shorten the heating time
[0005] Studies have shown that the aerobic fermentation process with cow dung as the main raw material has a slow temperature rise, especially at low temperatures in winter, and it is difficult to meet the requirements of harmlessness and maturity

Method used

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  • A kind of priming agent for aerobic fermentation of cow dung and preparation method thereof
  • A kind of priming agent for aerobic fermentation of cow dung and preparation method thereof
  • A kind of priming agent for aerobic fermentation of cow dung and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] (1) Mix Streptomyces xylophilus, Bacillus subtilis, Lactobacillus, Clostridium sporogenes, Thermomonospora, and Vibrio cellulosus according to the ratio of 25:15:5:20:17:18, and add them to the microbial culture Base (prepared by adding 5 g of beef extract, 15 g of peptone, and 5 g of sodium chloride to each 1 L of water, and sterilizing at 121°C for 25 minutes), cultured at 25°C for 30 hours, to obtain a composite microbial agent;

[0046] (2) Mix the volcanic rock, glucose and microbial culture medium uniformly at a ratio of 15g: 10ml: 70ml to obtain a high-efficiency immobilization medium.

[0047] (3) Take 1g of compound microbial agent and place it in a 250ml Erlenmeyer flask, add 10ml of high-efficiency immobilization medium to ensure that each gram contains 5×10 beneficial bacteria 8 Above, adsorb and fix in a 150rpm shaker at 25°C for 30h, centrifuge at 2000r / min for 15min, pour off the supernatant, and freeze-dry the lower layer to become the cow dung aerobic f...

Embodiment 2

[0049] (1) Mix Streptomyces xylophilus, Bacillus subtilis, Lactobacillus, Clostridium sporogenes, Thermomonospora, and Vibrio cellulosus according to the ratio of 27:15:5:18:20:15, and add them to the microbial culture (prepared by adding 5 g of beef extract, 15 g of peptone, and 5 g of sodium chloride to each 1 L of water, and sterilizing at 121° C. for 25 minutes), and cultured at 25° C. for 28 hours to obtain a composite microbial agent.

[0050] (2) Mix the volcanic rock, glucose and microbial culture medium uniformly in the ratio of 18g: 10ml: 70ml to obtain a high-efficiency immobilization medium.

[0051] (3) Take 1g of compound microbial agent and place it in a 250ml Erlenmeyer flask, add 8ml of high-efficiency immobilization medium to ensure that each gram contains 5×10 beneficial bacteria 8 Above, fix in 150rpm shaker at 25°C for 30h, centrifuge at 2000r / min for 25min, pour off the supernatant, and freeze-dry the lower layer to become cow dung aerobic fermentation pr...

Embodiment 3

[0053] (1) Mix Streptomyces xylophilus, Bacillus subtilis, Lactobacillus, Clostridium sporogenes, Thermomonospora, and Vibrio cellulosus according to the ratio of 25:15:5:20:25:10, and add them to the microbial culture (prepared by adding 5 g of beef extract, 15 g of peptone, and 5 g of sodium chloride to each 1 L of water, and sterilizing at 121° C. for 25 minutes), and cultured at 25° C. for 28 hours to obtain a composite microbial agent.

[0054] (2) Mix the volcanic rock, glucose and microbial culture medium uniformly in the ratio of 20g: 10ml: 75ml to obtain a high-efficiency immobilization medium.

[0055] (3) Take 1g of compound microbial agent and place it in a 250ml Erlenmeyer flask, add 6ml of high-efficiency immobilization medium to ensure that each gram contains 5×10 beneficial bacteria 8 As above, adsorb and fix in a 150rpm shaker at 25°C for 32h, centrifuge at 4000r / min for 15min, discard the supernatant, and freeze-dry the lower layer to become the priming agent...

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Abstract

The invention provides a primer for aerobic fermentation of cow dung and a preparation method thereof. The priming agent includes compound microbial bacterial agent, volcanic rock, glucose and microbial culture medium. The composite microbial bacterial agent is prepared by mixing different species of microbial bacterial agents such as Streptomyces xylophilus, Bacillus subtilis, Lactobacillus, Clostridium sporogenes, Thermomonospora, Vibrio cellulosus and the like. Aiming at the problems of high content of lignocellulosic substances in cow dung and low efficiency of aerobic fermentation, the present invention is based on the characteristics of changes in lignocellulosic substances and microbial community structures in the aerobic fermentation process of cow dung, combined with the principles of physics, chemistry and microbiology, to Inorganic conditioners, easily degradable carbon sources and compound microbial agents are used as raw materials to develop environmentally friendly, high-efficiency, and low-cost cow dung aerobic fermentation starters to accelerate the temperature rise of aerobic fermentation, prolong the high temperature period, and improve the quality and fermentation efficiency of aerobic fermentation products. The high-efficiency fertilizer utilization of cow dung provides technical support.

Description

technical field [0001] The invention belongs to the technical field of resource utilization of agricultural and rural waste, and in particular relates to a primer for aerobic fermentation with cow dung as the main raw material and a preparation method thereof. Background technique [0002] With the improvement of living standards, people's demand for dairy products and meat products has increased significantly, which has accelerated the large-scale development of my country's dairy and beef cattle breeding industry. Compared with other livestock and poultry farming industries, dairy and beef cattle farming produces the most manure and is the most difficult to deal with, which has become a bottleneck for the healthy and sustainable development of the farming industry. Due to the high feed digestibility of dairy cows, the high content of lignocellulosic substances in manure, and rich nitrogen and phosphorus nutrients, it is estimated that 1 ton of cow manure is equivalent to 2...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/20C05F3/00C12R1/465C12R1/125C12R1/225C12R1/145C12R1/63C12R1/01
CPCC12N1/20C05F17/20C05F3/00Y02W30/40Y02A40/20
Inventor 张曦沈玉君孟海波丁京涛赵立欣刘文杰周海宾程红胜
Owner ACADEMY OF PLANNING & DESIGNING OF THE MINIST OF AGRI
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