Lepista sordida strain and method for cultivating fruiting body by using liquid strain thereof

A technology of variegated mushrooms and liquid seeds, applied in the field of microorganisms, can solve the problems of long fruiting time, low yield, long cycle, etc.

Active Publication Date: 2020-10-09
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hu Xianyun et al. conducted liquid fermentation research on the mycelium of A. variegata, but did not cultivate fruiting bodies (Hu Xianyun; Jiang Jiazhi, Research on liquid fermentation of the mycelium of A. variegata, Food Research and Development, 2016, 37(15): 44-47 )
[0004] In addition, although the flower face mushroom has successfully produced mushrooms, the appearance of the mushrooms is uneven, the fruiting time is long, the yield is low and the cycle is long; and the cultivated species are all solid species, resulting in a small planting scale, which is far from meeting the market demand. See large-scale cultivation

Method used

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  • Lepista sordida strain and method for cultivating fruiting body by using liquid strain thereof
  • Lepista sordida strain and method for cultivating fruiting body by using liquid strain thereof
  • Lepista sordida strain and method for cultivating fruiting body by using liquid strain thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1. Isolation, Identification and Preservation of Lepista sordida DCH618CGMCC No.16980

[0083] 1. Isolation of the fungus DCH618

[0084] The inventor of the present invention collects wild fungus fruiting bodies from Dashiwo Town, Fangshan District, Beijing (see figure 1 , A is a side view, B is a top view). The tissue at the junction of the cap and the stipe was picked, placed on PDA medium, and cultured in the dark at 25° C. for 2 weeks to obtain mycelium. The hyphae were picked out and purified twice to obtain the fungus DCH618. The steps of each purification are as follows: the mycelium is placed on the PDA medium, and cultured in the dark at 25° C. for 2 weeks.

[0085] 2. Identification of the fungus DCH618

[0086] 1. Morphological identification

[0087] (1) Inoculate the mycelia of the fungus DCH618 on PDA medium (slant), culture in dark at 25° C. for 2 weeks, and observe the growth state of the mycelia.

[0088] See the experimental results fi...

Embodiment 2

[0099] Embodiment 2, the cultivation of the fruiting bodies of DCH618

[0100] 1. Cultivation process

[0101] The cultivation process of DCH618 fruiting body is as follows: preparation of mother seed → preparation of first-level liquid seed → preparation of second-level liquid seed (that is, cultivation liquid seed) → inoculation, film covering and germination → soil covering → fruiting management → Fruiting → Harvesting.

[0102] 1. Preparation of mother species

[0103] Inoculate the mycelium of DCH618 on the PDA medium (test tube slope), and cultivate in the dark at 25°C until the mycelia cover the medium, and the mycelium on the medium is the mother species.

[0104] 2. Preparation of primary liquid species

[0105] After completing step 1, transfer the mother species to the first-level liquid medium (the inoculum size is 8-10 mother species strains (about 1.0cm in diameter) per 200mL first-level liquid medium), at 25°C , 150rpm dark culture for 7-10d, to obtain a fir...

Embodiment 3

[0143] Example 3, High Performance Liquid Chromatography Analysis of DCH618 Fruiting Body

[0144] In this embodiment, the mobile phase for HPLC analysis is composed of 17 parts by volume of pH 6.5, 10 mM phosphate buffer and 3 parts by volume of methanol, the flow rate is 1.0 mL / min, and the column temperature is room temperature. The detection wavelength is 260nm.

[0145] 1. According to the cultivation process of step 1 in embodiment 2, obtain the flush mushroom, i.e. the fruiting body.

[0146] 2. After step 1 is completed, the fruiting bodies are taken, dried, and then ground to obtain fruiting body powder.

[0147] 3. After completing step 2, take a stoppered Erlenmeyer flask, add 0.5 g of fruiting body powder and 10 mL of 90% (v / v) methanol aqueous solution, seal it tightly, shake well, and heat to reflux for 30 min.

[0148] 4. After completing step 3, cool naturally, filter, and collect the filtrate.

[0149] 5. After completing step 4, take the test tube, add the f...

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Abstract

The invention discloses a Lepista sordida strain and a method for cultivating a fruiting body by using a liquid strain thereof. The invention provides Lepista sordida DCH618, and the preservation number of the Lepista sordida DCH618 is CGMCC No. 16980. Experiments prove that the fruiting body rich in nucleoside compounds and / or ergosterol can be cultivated by using the Lepista sordida DCH618 liquid strain, and large-scale industrialized cultivation can be realized. Therefore, the Lepista sordida DCH618 has important application value in food processing, medicine processing or health care product processing. The Lepista sordida strain and the method have important application value.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a method for cultivating sporophores of a genus chinensis strain and its liquid strain. Background technique [0002] The flower-faced mushroom is called the flower-faced mushroom because of the water-soaked pattern on the edge of the cap, also known as clove mushroom, flower-faced mushroom, purple flower-faced mushroom, etc. Mushrooms are important edible fungi in my country. Mushrooms are rich in protein, complete in amino acids, calcium, iron, zinc and other trace elements and vitamins necessary for the human body. [0003] It is a rare and high-quality wild edible fungus with strong aroma, crisp taste, pleasant color and high nutritional and economic value. But at present, the artificial cultivation of Amanita variegata is still in its infancy, and solid strains are mostly used. The output is low and the cycle is long, which is far from meeting the market demand. The paten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20A01G18/40A23L31/00A23L33/00A61K36/07A61P7/06A61P1/16A61P25/00A61P3/02C12N1/14C12R1/645
CPCA01G18/00A01G18/20A01G18/40A23L31/00A23L33/00A61K36/07A61P7/06A61P1/16A61P25/00A61P3/02C12N1/14C12R2001/645C12N1/145
Inventor 董彩虹刘晴刘奇正
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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