Multi-mode precisely-focused electrical impedance flow cytometry detection device and preparation method thereof

Abstract

The invention discloses a multi-mode precisely-focused electrical impedance flow cytometry detection device and a preparation method thereof. The device comprises a non-conductive body, and a hydrodynamic focusing flow channel, an ultrasonic focusing flow channel, an electrical impedance detection flow channel and an outlet flow channel are sequentially arranged in the body in the direction from the fluid inlet to the fluid outlet. The hydrodynamic focusing flow channel comprises a sample flow channel in the middle and a sheath fluid flow channel wrapping the sample flow channel; the sample flow channel is communicated with the sample inlet, the sheath fluid flow channel is communicated with the sheath fluid inlet through the sheath fluid flow inlet flow channel, a piezoelectric element isarranged on the outer side of the ultrasonic focusing flow channel, electrical impedance detection electrodes are arranged at the two ends of the electrical impedance detection flow channel, and double-nozzle 3D printing is adopted in the preparation method. According to the invention, the problem of congestion of a sample particle blocking point is solved, the targets of high focusing speed andaccurate focusing position are realized, rapid, accurate and effective focusing is carried out on cells, and the difficulty of signal processing is reduced.

Description

technical field [0001] The invention relates to a microfluidic detection device, in particular to a multi-mode precise focusing electrical impedance flow cytometric detection device and a preparation method. Background technique [0002] Microfluidic devices are usually also called microfluidic chips, which are mainly used in chemical analysis, biomedicine, environmental monitoring and other fields. Combined with flow cytometry, it is often used to detect particles such as cells, genetic material, and proteins. During detection, the sample particles in the micro-channel should be stably focused at the center to improve detection accuracy. The methods of focusing mainly include methods such as hydrodynamic focusing and acoustic focusing, and the methods of detection mainly include methods such as electrical impedance detection, fluorescence detection, light scattering, and microscopic imaging. [0003] The hydrodynamic focusing method exerts a large focusing force on the ce...

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Problems solved by technology

[0005] Purpose of the invention: The purpose of the present invention is to provide a multi-mode precise focusing electrical impedance flow cytometry detection device and preparation method that solves the problems of single detection mode, inaccurate focus position and blockage of sample particles

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