A microfluidic chip for monitoring nematode motility behavior and physiological characteristics

Abstract

The invention discloses a microfluidic chip for monitoring nematode movement behavior and physiological characteristics, wherein: a fluid inlet, a culture chamber array, a sampling channel, an electrical impedance tomography detection chamber, an electrical impedance spectrum detection chamber and a fluid outlet are sequentially connected The sampling control valve is set on the sampling channel for controlling the conduction or closing of the channel; the detection control valve is set between the electrical impedance tomography detection chamber and the electrical impedance spectroscopy detection chamber for controlling the conduction or closure of the channel Closed; the electrical impedance tomography electrode array is connected to the electrical impedance tomography detection cavity; the electrical impedance spectrum detection electrode array is connected to the electrical impedance spectrum detection cavity. Using the above technical scheme, the integration of the culture chamber, electrical impedance tomography detection chamber and electrical impedance spectroscopy detection chamber can be realized, and the functions of nematode cultivation, movement behavior and physiological structure detection can be realized. Combined with the control valve, high automation, Efficient cultivation and detection.

Description

technical field [0001] The invention relates to the field of microfluidic chips, in particular to a microfluidic chip used for monitoring the movement behavior and physiological characteristics of nematodes. Background technique [0002] Microfluidics Chip (Microfluidics Chip) is a cutting-edge technology for fluid manipulation by manufacturing submicron-scale microchannels and chamber networks through micromachining technology, and integrates operations in traditional biochemical analysis laboratories on tiny chips. The intersection of microfluidic chip technology and fluid mechanics, electromagnetics, acoustics, optics and other disciplines has been widely used in the research of biology and chemistry. Nematodes are commonly used model organisms in biological research. [0003] The microfluidic chip used for nematode biology research in the prior art lacks the ability to detect the movement behavior and physiological structure of nematodes. Nematode research in the prior...

AI Technical Summary

Problems solved by technology

Nematode research in the prior art uses platinum probes on the surface of the agarose gel, which has low work efficiency, difficult control of the culture environment, and few parameters that can be measured simultaneously

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