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Fermentation culture medium for bacillus subtilis, preparation method for fermentation culture medium, and preparation method for bacillus subtilis preparation

A technology of Bacillus subtilis and fermentation medium, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low number of viable bacteria in fermentation broth, long fermentation culture time, low spore rate, etc. Achieve the effects of increasing fermentation temperature, improving nutrient content and utilization efficiency, and improving utilization rate

Active Publication Date: 2020-10-02
HENAN JINBAIHE BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of fermentation medium for Bacillus subtilis, to solve the problem of low number of viable bacteria and low spore rate in the fermented liquid obtained from the fermentation medium for Bacillus subtilis
[0007] Second object of the present invention is to provide a kind of preparation method of Bacillus subtilis fermentation medium, to solve the low number of live bacteria when the Bacillus subtilis fermentation medium obtained by the existing preparation method is used for Bacillus subtilis fermentation culture , The problem of low spore rate
[0008] The third object of the present invention is to provide a preparation method of Bacillus subtilis preparation to solve the problems of long fermentation and cultivation time, poor stability, low number of viable bacteria in the fermentation liquid and low spore rate in the existing preparation method

Method used

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  • Fermentation culture medium for bacillus subtilis, preparation method for fermentation culture medium, and preparation method for bacillus subtilis preparation
  • Fermentation culture medium for bacillus subtilis, preparation method for fermentation culture medium, and preparation method for bacillus subtilis preparation
  • Fermentation culture medium for bacillus subtilis, preparation method for fermentation culture medium, and preparation method for bacillus subtilis preparation

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Effect test

Embodiment 1

[0034]The fermentation medium of this embodiment consists of: soybean meal powder 72g / L, cornstarch 88g / L, corn steep liquor 5g / L, beef extract 2.6g / L, peptone 11.4g / L, sodium chloride 3.2g / L, glucose 10.2g / L, calcium carbonate 4.5g / L, dipotassium hydrogen phosphate 1.0g / L, ferric chloride 0.5g / L, manganese sulfate 0.05g / L, protease (2000U / g) 2.8g / L, amylase ( 2000U / g) 2.8g / L, wherein, the dry matter content in the corn steep liquor is 47.3%, and the total nitrogen content is 3.47%.

Embodiment 2

[0036] The fermentation medium of the present embodiment consists of: soybean meal powder 64g / L, cornstarch 84g / L, corn steep liquor 6.8g / L, beef extract 2.2g / L, peptone 11.6g / L, sodium chloride 3.8g / L, Glucose 12.0g / L, calcium carbonate 4.8g / L, dipotassium hydrogen phosphate 0.9g / L, ferric chloride 2.2g / L, manganese sulfate 0.04g / L, protease (2000U / g) 2.2g / L, amylase (2000U / g) 2.2g / L, wherein, the dry matter content in the corn steep liquor is 49.0%, and the total nitrogen content is 3.62%.

Embodiment 3

[0038] The fermentation medium of the present embodiment consists of: soybean meal powder 70g / L, cornstarch 83g / L, corn steep liquor 5.5g / L, beef extract 2.7g / L, peptone 8.1g / L, sodium chloride 2.2g / L, Glucose 8.4g / L, calcium carbonate 3.7g / L, dipotassium hydrogen phosphate 0.7g / L, ferric chloride 0.7g / L, manganese sulfate 0.06g / L, protease (1800U / g) 2.9g / L, amylase (1800U / g) 3.0g / L, wherein, the dry matter content in the corn steep liquor is 48.0%, and the total nitrogen content is 3.52%.

[0039] Two, the specific embodiment of the preparation method of Bacillus subtilis fermentation medium of the present invention

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Abstract

The invention belongs to the field of microbial fermentation, and specifically relates to a fermentation culture medium for bacillus subtilis, a preparation method for the fermentation culture medium,and a preparation method for a bacillus subtilis preparation. The fermentation culture medium is prepared from 63-74 g / L of bean pulp powder, 82-90 g / L of corn starch, 5-7 g / L of corn steep liquor, 2-3 g / L of a beef extract, 8-12 g / L of peptone, 8-12 g / L of glucose, 2-3 g / L of protease, 2-3 g / L of amylase and 5.73-11.08 g / L of inorganic salt, wherein the enzyme activity of the protease is 1800-2000 U / g; and the enzyme activity of the amylase is 1800-2000 U / g. The fermentation culture medium provided by the invention can provide a nutritional environment applicable to growth of the bacillus subtilis, and the viable count and spore count in a fermentation broth are increased.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a fermentation medium for bacillus subtilis and a preparation method thereof, and a preparation method for a bacillus subtilis preparation. Background technique [0002] Bacillus subtilis is a kind of Bacillus genus, which is widely distributed in soil and decayed organic matter, and is named after it is easy to reproduce in the withered grass juice. Bacillus subtilis has no capsule, has flagella, can move, Gram staining is positive, spores ((0.6~0.9)μm×(1.0~1.5)μm) are located in the center of the thallus or slightly biased, and the bacterium is not formed after spore formation Swelling; the surface of the colony is rough and opaque, stained white or yellowish, and often forms wrinkled walls when growing in liquid medium, which is an aerobic bacteria; it can use protein and various sugars to decompose tryptophan. Bacillus subtilis is one of the probiotics appro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/125
CPCC12N1/20
Inventor 朱广跃李克克谢璐璐冯利菲李光华韩亚坤买超梁韩娅楠
Owner HENAN JINBAIHE BIOTECH CO LTD
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