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CPR kit with function of detecting gene mutation of human IDH1 or IDH2

A technology of IDH2R172K and IDH1, which is applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of low detection sensitivity and long detection cycle, and achieve high specificity and high accuracy.

Inactive Publication Date: 2020-09-25
上海佰臻生物科技有限公司
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

After decades of clinical application, it has been recognized by the majority of medical staff, but the detection sensitivity of this technology is relatively low, and the detection cycle is long

Method used

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  • CPR kit with function of detecting gene mutation of human IDH1 or IDH2
  • CPR kit with function of detecting gene mutation of human IDH1 or IDH2

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Embodiment 1

[0032] Such as Figure 1-2 As shown, the present invention provides a PCR kit for detecting human IDH1 or IDH2 gene mutations. The kit includes a nucleic acid amplification reagent and a reference substance. The nucleic acid amplification reagent includes an IDH1R132H reaction solution and an IDH2R172K reaction solution. Contains specific primers and probes for detecting IDH1R132H and IDH2R172K mutations. Each reaction solution contains a pair of upstream primers and downstream primers, and TaqMan fluorescent probes. The primer probe sequences and modifications are as follows:

[0033] IDH1R132H reaction solution: upstream primer 5’-GATTTTGGTCTAGCTACAGA-3’

[0034] Downstream primer 5’-CACAAAATGGATCCAGAC-3’

[0035] Fluorescent probe 5’-FAM-GAAATCTCGATGGAGTGGGTCC-TAMRA-3’;

[0036] IDH2R172K reaction solution: upstream primer 5’-GATTTTGGTCTAGCTATAAA-3’

[0037] Downstream primer 5’-CACAAAATGGATCCAGAC-3’

[0038] Fluorescent probe 5’-FAM-GAAATCTCGATGGAGTGGGTCC-TAMRA-3’;

[0039] In addit...

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Abstract

The invention discloses a PCR kit with a function of detecting gene mutation of human IDH1 or IDH2. The kit comprises a nucleic acid amplification reagent and reference substance, wherein the nucleicacid amplification reagent is prepared from an IDH1R132H reaction liquid and an IDH2R172K reaction liquid; the reaction liquids respectively contain specific primers and probes used for detecting mutation of IDH1R132H and IDH2R172K; and each reaction liquid contains a pair of upstream primer and downstream primer. The kit adopts the allele specific amplification quantitative PCR technology, can perform qualitative detection on mutation of IDH1R132H and IDH2R172K so as to assist glioma clinical diagnosis and guiding related patients for treatment and detection, and has high specificity, high accuracy and high sensitivity.

Description

Technical field [0001] The present invention relates to the field of gene detection, in particular to a PCR kit for detecting human IDH1 or IDH2 gene mutations. Background technique [0002] IDH is a family of enzymes that play an important role in the tricarboxylic acid cycle. These enzymes participate in the tricarboxylic acid cycle and catalyze the production of α-ketoglutarate (α-KG) from isocitrate through oxidative decarboxylation. In aerobic organisms, the tricarboxylic acid cycle is part of the metabolic pathway, through the chemical conversion of carbohydrates, proteins, and fats to form carbon dioxide, water and energy required by the body. The two coenzymes of IDH, nicotinamide adenine dinucleotide phosphate (NADP) and nicotinamide adenine dinucleotide (NAD) are closely related to the activity of IDH. Human genes have 5 IDH genes (IDH1 and IDH2 are homologous genes) encoding 3 different IDH enzymes, which can be divided into NADP-dependent (IDH1 and IDH2) and NAD-depe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6886C12Q2600/156C12Q2600/166C12Q2531/113C12Q2561/101C12Q2521/101C12Q2563/107
Inventor 陆军
Owner 上海佰臻生物科技有限公司
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