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Pseudomonas chlororaphis subsp.aurantiaca as well as preparation and application of microbial agent thereof

A technology of Pseudomonas chloropinus and microbial agent, applied in the field of microorganisms, can solve problems not involved in the prevention and control of fungal diseases and other various pests, and achieve the effects of improved safety, good effect and environmental protection

Active Publication Date: 2020-09-25
山东五福生生态工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent document CN109258695A (application number: 201811214677.X) discloses the application of Pseudomonas chloropinus in the prevention and treatment of nematodes. The application of fungicides, but not involved in the control of fungal diseases and other various pests
[0006] Chinese patent document CN107099474A (application number: 201710333734.5) discloses a Pseudomonas chloropinus with broad-spectrum antibacterial activity and its application. It has a biological control effect on apple ring spot, cherry blight, grape anthracnose, grape downy mildew, and wheat sheath blight, but it does not involve the application of controlling crop pests

Method used

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  • Pseudomonas chlororaphis subsp.aurantiaca as well as preparation and application of microbial agent thereof
  • Pseudomonas chlororaphis subsp.aurantiaca as well as preparation and application of microbial agent thereof
  • Pseudomonas chlororaphis subsp.aurantiaca as well as preparation and application of microbial agent thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0067] The screening of Pseudomonas chloropinus subspecies orange-yellow subspecies 2501 comprises the following steps:

[0068] Isolation of bacteria in Shouguang Vegetable Protected Land: KMB, LB medium, and nutrient agar medium were used to isolate bacteria from soil, roots and stems of healthy plants, and a total of 126 bacterial strains were isolated.

[0069] 1. Taking plant pathogenic fungi as targets to further screen the isolated and purified bacteria

[0070] The tested phytopathogenic fungi (Fusarium oxysporum, Phytophthora, and Sclerotinia) stored in a refrigerator at 4°C were transferred to PDA plates for activation, cultured at 30°C for 3 days, and plate confrontation method was used to protect vegetables from Shouguang. Bacteria isolated and purified from the ground were used as targets to test the phytopathogenic fungi to determine their antibacterial activity. The specific method is: after the pathogenic bacteria are overgrown on the plate, punch out the bact...

Embodiment 1-2

[0083] Morphological characteristics of the 2501 strain: Gram staining was negative. On the KMB solid medium, the colonies were round and flat, and the colonies were moist. They produced green pigments at the initial stage of cultivation, and orange-yellow pigments at the later stage of cultivation. They were Pseudomonas chloropinus Pseudomonas chlororaphis subsp. aurantiaca, named Pseudomonas chlororaphis subsp. aurantiaca 2501.

[0084] The morphological characteristics of the 1018 strain: on the KMB medium, the colonies are round and flat, the colonies are moist, and the Gram staining is negative. The green pigment is produced at the early stage of the culture, and the orange-yellow pigment is produced at the later stage of the culture. Compared with 2501, the same culture Time, the colony should be small, for Pseudomonas chlororaphis (Pseudomonas chlororaphis), named Pseudomonas chlororaphis 1018.

[0085] The above-mentioned Pseudomonas chlororaphis subsp. aurantiaca 2501...

Embodiment 2-1

[0087] 2501 A method for preparing a fermented product, comprising the steps of:

[0088] (1) Strain activation: Pseudomonas chloropinus subsp. orange 2501 was activated on a solid medium, the culture temperature was 30±0.5°C, and the culture time was 48 hours;

[0089] The components of the solid medium are as follows: tryptone 10g / L, yeast powder 5g / L, sodium chloride 10g / L, glucose 5g / L, agar 17g / L, balance water, pH 7.2-7.4.

[0090] (2) Preparation of primary seed liquid: inoculate the activated bacteria in step (1) in primary liquid seed medium, culture temperature 30±0.5°C, rotation speed 190-200rpm, culture time 22-24h, and obtain a grade seed liquid;

[0091] The components of the primary liquid seed medium are as follows: tryptone 10g / L, yeast powder 5g / L, sodium chloride 10g / L, glucose 5g / L, balance water, pH 7.2-7.4.

[0092] (3) Preparation of the secondary seed liquid: inoculate the primary seed liquid prepared in step (2) into the secondary seed liquid medium, t...

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Abstract

The invention discloses a pseudomonas chlororaphis subsp.aurantiaca as well as preparation and application of a microbial agent thereof. The pseudomonas chlororaphis subsp.aurantiaca 2501 disclosed bythe invention not only has a sterilization function, but also has an insecticidal function, and fills the blank that pseudomonas chlororaphis found at present only has a single biocontrol function. The invention also discloses a liquid microbial agent II of the pseudomonas chlororaphis subsp.aurantiaca 2501. The liquid microbial agent II comprises the following components: Tween 80, trehalose, sucrose, orange peel essential oil and a 2501 liquid microbial agent I; a solid microbial agent II of the pseudomonas chlororaphis subsp.aurantiaca 2501 comprises the following components: glucose, beanpulp powder, potassium fulvate and a 2501 solid microbial agent I; and the remarkable application effect is achieved through a field experiment.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to the preparation and application of Pseudomonas chloropinus subsp. orange and a microbial bacterial agent thereof. Background technique [0002] At present, there are many kinds of microbial agents used for the biological control of crop diseases and insect pests, and the use effects are uneven, and most of the microbial agents used for biological control have a single effect on the control of crop diseases and insect pests, and can only be used to control fungal diseases or can only be used There are very few types of microbial inoculants for preventing and controlling single pests, and simultaneously for the prevention and treatment of fungal diseases and insect pests, and there are very few types of microbial inoculants for simultaneously preventing and controlling multiple fungal diseases and various insect pests. [0003] Fungal diseases and insect pests of crops...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/27A01N25/02A01N25/08A01P3/00A01P7/04C12R1/38
CPCC12N1/20A01N63/27A01N25/08
Inventor 刘峰潘明王江伟陈小奇
Owner 山东五福生生态工程有限公司
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