Preparation and application of thrombolytic enzyme
A technology of thrombolytic enzyme and fibrinolysis, applied in the field of thrombolytic enzyme, can solve problems such as being in the experimental stage, and achieve the effects of high-efficiency dissolution, significant effect, and blood supply recovery.
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[0026] The present invention will be further described according to the following examples, and the mode of the present invention includes but not limited to the following examples.
[0027] The strains, vectors, and media used in this example are Escherichia coli Trelief 5a-pClone007 and BL21(DE3)-pDE1 for cloning and overexpression respectively. Escherichia coli was cultured in LB medium with shaking at 37°C. LB selection medium added with 50 μg / ml kana is used for screening, cultivating recombinant Escherichia coli and producing recombinant protein.
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