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Application of exosome in preparation of drugs or cosmetics for repairing skin injury

A technology for repairing skin damage, exosomes, used in the field of application in medicine or cosmetics

Active Publication Date: 2020-09-18
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at how to solve the related problems of skin damage repair, the present invention provides a fast and convenient technical method for preparing exosomes from velvet antler mesenchymal stem cells and its application in the process of skin damage repair

Method used

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  • Application of exosome in preparation of drugs or cosmetics for repairing skin injury
  • Application of exosome in preparation of drugs or cosmetics for repairing skin injury
  • Application of exosome in preparation of drugs or cosmetics for repairing skin injury

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Example 1. Preparation method of exosomes derived from velvet antler mesenchymal stem cells.

[0041] 1) Primary isolation and culture of velvet antler mesenchymal stem cells: After pretreatment of velvet antler tissue, the mesenchymal area is obtained, and mesenchymal stem cells are isolated in a sterile environment; the specific operation method is as follows:

[0042] Fresh velvet antler is collected at the end of spring and the beginning of summer, and the antler mesenchymal zone is the tissue of fresh velvet antler, which is located at the tip of the velvet antler.

[0043] Pretreatment operation: First, saw off the whole piece of velvet antler, squeeze out the excess blood, put it in tin foil, seal it, put it in a foam box with ice packs, and send it back to the laboratory in time. Separate the tip of the velvet tissue in a sterile ultra-clean bench, take the tip of the velvet tissue about 3cm in length, scrape off the outer fluff of the skin tissue with a sterili...

Embodiment 2

[0056] Example 2. The role of antler-derived mesenchymal stem cells (AMSCs) exosomes (Exo) in the treatment of skin lesions.

[0057] Taking mice as experimental subjects, the effect of the antler mesenchymal stem cells (AMSCs) exosomes prepared in Example 1 on the healing of skin injuries in mice was explored.

[0058] 1. Experimental animals and grouping:

[0059] (1) Construct an animal model of skin wound

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Abstract

The invention discloses an application of an exosome in preparation of drugs or cosmetics for repairing skin injury, belonging to the technical field of skin treatment. In order to solve the problem of skin injury, the exosome provided by the invention is obtained through the following steps: separating mesenchymal stem cells from primary cornu cervi pantotrichum tissues; and carrying out in-vitroamplification culture, collecting a cell culture solution, and carrying out separation and extraction so as to obtain an antler mesenchymal stem cell-derived exosome. The exosome provided by the invention is proved to be able to inhibit LPS induced inflammatory response of HaCat cells through an NLRP3 inflammasome signaling pathway, accelerate wound repair of a mouse model with skin injury and relieve inflammatory response. The exosome provided by the invention can be used for preparing the drugs or the cosmetics for treating skin injury repairing.

Description

technical field [0001] The invention belongs to the technical field of skin treatment, and in particular relates to the application of exosomes in the preparation of medicines or cosmetics for repairing skin damage. Background technique [0002] The skin is the largest organ of the human body. As the protective barrier of the human body, it plays an important role in protecting the body from external mechanical damage and preventing pathogenic microorganisms and chemical substances from invading the body. Skin damage includes mechanical damage and pathological damage caused by external forces, such as: skin damage in daily life, chronic non-healing pathological damage caused by complications of diabetic patients. Studies have shown that inhibiting inflammation during skin injury repair is the key to promoting skin injury repair and healing. Inflammation is the activation of the innate immune system in response to invading pathogens attacking skin tissue. Current research r...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P17/00A61P17/02A61K8/99A61K8/98A61Q19/00C12N5/0775
CPCA61K8/985A61K8/99A61K35/28A61Q19/00A61P17/00A61P17/02C12N5/0668C12N2509/00C12N2509/10
Inventor 杨春张琦孙胜楠郭佳彭英华
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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