Bacillus licheniformis and screening method and application thereof

A technology of Bacillus licheniformis and Bacillus licheniformis, applied in the field of microorganisms, to achieve the effects of reducing production costs, high enzyme activity, and realizing resource utilization

Active Publication Date: 2020-09-04
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report of using bacillus licheniformis to prepare bio-organic fertilizer by using distiller's grains

Method used

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  • Bacillus licheniformis and screening method and application thereof
  • Bacillus licheniformis and screening method and application thereof
  • Bacillus licheniformis and screening method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0033] A kind of Bacillus licheniformis in the present invention, classified and named as Bacillus licheniformis JZLGZ-1, the preservation number is CGMCC No.18747, the preservation date is October 28, 2019, and the preservation unit is China Microorganism Culture Preservation Management Committee Ordinary Microbiology Center, the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

Embodiment 2

[0035] The nucleotide sequence of Bacillus licheniformis is shown as SEQ ID No: 1, and the screening steps are as follows:

[0036] (1) Take samples in the brewing workshop of the liquor factory, select the discarded liquor after distillation and throw it away, and put it into a sterile sampling bag.

[0037] (2) Weigh 10g of distilled spirits and place in a 100mL Erlenmeyer flask, add 90mL of sterile saline, and shake well;

[0038] (3) Take the supernatant of the solution after mixing and dilute it successively to 10 times by 10 times dilution method. -2 、10-3 、10 -4 times the diluted solution;

[0039] (4) Use a sterile pipette gun to pipette 100 μL of each dilution and apply it to 30 mL of Congo red culture medium, and spread the bacterial solution evenly on the surface of the medium with a coating stick; The surface of the rear medium is not easy to dry, and it is easy to cause bacterial contamination when inverted;

[0040] (5) Place in a constant temperature incubat...

Embodiment 3

[0047] The screening steps of Bacillus licheniformis strains are as follows:

[0048] (1) Take samples in the brewing workshop of the liquor factory, select the discarded liquor after distillation and throw it away, and put it into a sterile sampling bag.

[0049] (2) Weigh 10g of distilled spirits and place in a 100mL Erlenmeyer flask, add 90mL of sterile saline, and shake well;

[0050] (3) Take the supernatant of the solution after mixing and dilute it successively to 10 times by 10 times dilution method. -2 、10 -3 、10 -4 times the diluted solution;

[0051] (4) Use a sterile pipette gun to pipette 100 μL of each dilution solution into the Congo red medium, and spread the bacterial solution evenly on the surface of the medium with a coating stick;

[0052] (5) placed in a constant temperature incubator at 37°C for 2 days;

[0053] (6) Select the bacterium that is a single colony in the above-mentioned medium, and pick the bacterium that produces the hydrolysis circle r...

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Abstract

The invention relates to the field of microorganisms, in particular to bacillus licheniformis and a screening method and application thereof. The bacillus licheniformis used in the invention is screened from distiller's grains of Chinese liquor, has a collection number of CGMCC No.18747, and is collected in the China General Microbiological Culture Collection Center on October 28, 2019. The bacillus licheniformis is easy to culture and relatively high in enzyme activity. In bio-organic fertilizer prepared by fermenting the distiller's grains of the Chinese liquor with the bacillus licheniformis, the content of organic matters (based on dry basis) exceeds 90%, the effective viable count (CFU) reaches 0.7-1.1 hundred million/g, the pH value is after fermentation is 5.5-6.5, and the water content is 15%-20%, which are all in the international standard ranges (according to the requirements of the national standard, the content of the organic matters (based on dry basis) is larger than or equal to 40.0%, the effective viable count (CFU) is larger than or equal to 0.20 hundred million/g, the water content is smaller than or equal to 30.0%, and the pH value is 5.5-8.5); and the bacillus licheniformis has relatively high application value.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a bacillus licheniformis and its screening method and its application in preparing bio-organic fertilizer by using distilled grains. Background technique [0002] Waste grains are one of the most important by-products of liquor production. With the increase of liquor production, the output of waste grains is also increasing. The production of 1,000 liters of liquor will produce about 8 tons of waste grains. If it is not handled properly or discarded directly, it will inevitably cause environmental pollution. It is also used to directly make compost and feed, but the utilization rate is not high, resulting in waste of resources. How to make good use of waste has become an urgent problem to be solved in the liquor industry. Waste grains are rich in nutrients, mainly containing cellulose, protein and starch, among which cellulose accounts for 17-21%, and the cellulose content is very high....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/34C12Q1/04C05F17/20C12R1/10
CPCC12N1/20C12N1/02C12Q1/34C12Q1/04C12R2001/10C12N1/205Y02W30/40
Inventor 赵长青蒋佳林高子晴罗路南罗惠波赵阳
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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