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Biomagnetism separation method based on movable magnetic net

A magnetic separation and magnetic mesh technology, applied in biochemical equipment and methods, methods of supporting/immobilizing microorganisms, methods of stress-stimulating microbial growth, etc., can solve the problems of cumbersome operation process, time-consuming and laborious separation efficiency, etc. Separation effect, good separation, good adsorption and release effect

Pending Publication Date: 2020-09-04
NANJING UNIV
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Problems solved by technology

[0005] The first object of the present invention is to provide the biological magnetic separation method based on mobile magnetic network, to solve the biological material separation process in the prior art (comprising adopting magnetic bead method to carry out the separation of cell, virus, protein, nucleic acid etc.) The technical problems of cumbersome operation process, time-consuming and labor-intensive and poor separation efficiency

Method used

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  • Biomagnetism separation method based on movable magnetic net
  • Biomagnetism separation method based on movable magnetic net
  • Biomagnetism separation method based on movable magnetic net

Examples

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example

[0027] Example: Using this method to magnetically separate exosomes labeled with immunomagnetic beads, the specific steps are:

[0028] (1) The immunomagnetic bead-labeled exosomes were dissolved in buffer.

[0029] (2) The magnetic field is provided by the magnetic rod in the magnetic sleeve, so that the magnetic net is magnetic.

[0030] (3) By rotating the magnetic net at a certain speed (generally 5r / min-500r / min), the exosomes labeled with magnetic beads in the adsorption suspension are captured and separated.

[0031] (4) Remove the buffer in the container and add new buffer.

[0032] (5) By making the magnetic bar leave the magnetic sleeve, the magnetic net is not in the magnetic field and does not have magnetism.

[0033] (6) By rotating the magnetic net at a certain speed (generally 5r / min-500r / min), the exosomes labeled with magnetic beads adsorbed by the magnetic net are released and suspended in a new buffer.

[0034] (7) Pipette the solution into a new containe...

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Abstract

The invention relates to a biomagnetism separation method based on a movable magnetic net, and belongs to the technical field of biology. The biomagnetism separation method is used for separating or purifying biology objects labelled magnetically or labelled with magnetic beads. Through new design of the movable magnetic net, the gradient of magnetic field intensity closed to an acquisition targetis notably increased, and the catching capacity is improved. The required core components comprise the magnetic net (3), magnetic rods (1), magnetic sleeves (2), and a suspension biology object solution storing container (4). The magnetic net is a super paramagnetism film, and is magnetic in a magnetic field and is antimagnetic when being divorced from the magnetic field. The existence of the magnetic field is controlled through the situation whether the magnetic rods are in the magnetic sleeves or not,the magnetic net rotates to enable each object labelled by magnetic beads in the storing container to have high magnetic field intensity gradient, and high separation effect is realized. The magnetic net rotates under the condition of being nonmagnetic to release the objects. The separationmethod provided by the invention is suitable for separation of different amount of biology objects (exosomes, nucleic acids, proteins and the like ) by a magnetic bead method.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a biomagnetic separation method based on a moving magnetic net. Background technique [0002] The sorting of biological cells and biomolecules is a common requirement in many biotechnology industries. Important biological cell sorting objects include exosomes, stem cells, circulating tumor cells, etc.; important biomolecular sorting objects include nucleic acids, proteins, etc. Sorting based on magnetic force is a major biological sorting method, which realizes the sorting of microscopic objects through the cooperation of magnetic beads and magnetic equipment. [0003] At present, most of the magnetic separation methods on the market use the strong magnetic field strength gradient near the magnet to passively adsorb and separate the target objects, and the magnetic rack method is more commonly used. For the magnetic rack method, the principle is to first allow the separ...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/12C12M1/00C07K1/14
CPCC12M21/02C12M47/04C12M35/06C07K1/14
Inventor 阮刚陈彦名窦亭玉陈阮晴雅克·路易徐静雯张蕾
Owner NANJING UNIV
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