Method for detecting early pregnancy of sheep

Abstract

The invention relates to a method for detecting early pregnancy of sheep. The method comprises the following steps of: collecting sheep blood or urine from the seventh day to the fourteenth day aftermating of ewes; inserting sheep chorionic gonadotropin HCG detection test paper into serum or urine for 5-10 seconds, and after 15-18 minutes, indicating that a detection result is invalid if a test paper quality control line C does not develop color and a detection line T develops color; indicating that an HCG level of ewes is low if the quality control line C develops color and the detection line T does not develop color; comparing developed colors with an HCG colorimetric card if the quality control line C and the detection line T both develop colors; indicating that the ewes are not pregnant if HCG is smaller than 10 mIU / ml; indicating that the ewes are possibly pregnant and need to be continuously detected twice if the HCG is greater than or equal to 10 mIU / ml, and indicating that theewes are pregnant and have living embryos if the HCG of twice detection rises and the HCG detected later is 1-2 times of the HCG detected last time; and indicating that ewes are pregnant but encounter embryo damage if the HCG of twice detection is kept unchanged or decreased, and taking miscarriage prevention measures or stopping pregnancy.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for detecting early pregnancy of sheep, and belongs to the technical field of animal detection methods. Background technique [0002] At present, the pregnancy detection of sheep is mainly done through the external observation method and the test method, that is, 17 to 21 days after the ewe is mated, observe whether the ewe returns to oestrus to judge whether the ewe is pregnant, but in actual observation, when The sheep embryo stops developing, and the ewe will not be in estrus in a short period of time, that is, it is in a state of false pregnancy. Existing technologies also include ultrasonic detection, immunological detection, red blood cell agglutination detection, serum acid titration detection, etc. Among them, ultrasonic detection uses the physical characteristics of ultrasonic waves to closely combine the physical characteristics of ultrasonic waves with the acoustic charac...

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Problems solved by technology

Existing technologies also include ultrasonic detection, immunological detection, red blood cell agglutination detection, serum acid titration detection, etc. Among them, ultrasonic detection uses the physical characteristics of ultrasonic waves to closely combine the physical characteristics of ultrasonic waves with the acoustic characteristics of animal tissue structures. This method needs 40 days after the sheep are mated to detect whether the sheep is pregnant, and the accuracy rate can only reach 100% after 60 days; the immunological detection is to use the hormones and some unknown substances in the serum, feces and urine of ewes before and after pregnancy to detect, according to Its content can be used to judge whether ewes are pregnant. Because these hormones in the serum of pregnant and non-pregnant sheep will be affected by factors such as breed and individual differences, it is still difficult to promote them in production; The specific antigen in the body is combined with red blood cells, and it is used to prepare antiserum. When mixed with red blood cells of ewes who are pregnant for 10-15 days, red blood cell agglutination will occur. If not pregnant, red blood cell agglutination will not occur. The accuracy rate of 28-60 days of sheep pregnancy is 90%, but red blood cell agglutination will also occur after the ewe embryo stops developing, which will easily lead to the long-term false pregnancy state of the ewe; During the process, the body produces a unique immunoglobulin, which forms low-soluble complexes (flocs) with other proteins in an acidic environment, and the accuracy rate can reach 90.81%. The characteristic of forming low-soluble complexes (flocs) with other proteins will still cause the ewes to be in a state of false pregnancy. At the same time, the operation process is cumbersome and needs to be carried out at a room temperature of 18--25 degrees, which is not conducive to the on-site detection

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