Method for detecting and counting biomass of acaudina molpadioides larvae in seawater

Abstract

A method for detecting and counting the biomass of acaudina molpadioides larvae in seawater comprises the following steps of: extracting acaudina molpadioides adult DNA, and sequencing to obtain a COIgene sequence; manufacturing standard substances with four concentration gradients by taking a PCR product as an initial standard substance; designing and verifying fluorescent quantitative PCR specific primers, and obtaining a standard curve of CT value-DNA concentration logarithm values after verification is passed; extracting DNA from acaudina molpadioides tissues with different dry weights, and performing fluorescent quantitative PCR quantitative test by taking the DNA of the acaudina molpadioides tissues with different dry weights as a template, so that a trend linear equation of DNA quality and acaudina molpadioides tissue dry weights is obtained; measuring a CT value of DNA in a seawater sample to be measured, substituting the CT value into a acaudina molpadioides tissue dry weight-CT value derivation relational expression, so that a concentration value of acaudina molpadioides larva DNA is obtained, and calculating to obtain the DNA mass of the acaudina molpadioides larva; andcalculating the wet weight of the acaudina molpadioides larva through the dry-wet weight ratio and the derivation relational expression.

Description

technical field [0001] The invention belongs to the field of biomass statistics of plankton, in particular to a method for statistics of biomass of sweet potato larvae in sea waters of nuclear power plants. Background technique [0002] Sea sweet potato (Acaudina spp) belongs to the phylum Echinodermata, the class Holothuroidea, the subclass Holothuroidea, the order Molpadida, the family Caudinidae, and the genus Acaudina. There are two species in this genus - Acaudina leucoprocta H.L. Clark and Acaudina molpadioidea Semper. Commonly known as sea eggplant, it is rich in subtropical sandy sea areas, Japan, the Philippines, Indonesia, and my country's Hainan, Guangdong, Fujian, and Zhejiang coasts. At present, the research on Haitian melon is mainly focused on the nutritional value, medicinal value and physiological function of Haitian melon. There are few reports on the biomass statistics of Haitian melon. Tang Yafei and other studies have shown that Haitian melon can cause ...

AI Technical Summary

Problems solved by technology

At present, the biomass statistics of plankton are mainly for a certain type, and there is still a lack of estimation methods for the biomass of a certain type of plankton in sea areas.

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