Terahertz imaging-based biomacromolecule content relative quantitative detection method

Abstract

The invention relates to a terahertz imaging-based biomacromolecule content relative quantitative detection method, and belongs to the field of biomacromolecule detection. The method comprises the following steps: S1, obtaining a nitrocellulose film or a polyvinylidene fluoride film containing biomacromolecules; S2, placing the to-be-detected film on a terahertz time-domain spectrometer, and carrying out scanning imaging to obtain a time-domain spectrum of a point; S3, performing Fourier transform and smoothing processing on the time domain spectral data, and taking the processed time domain spectral data as frequency domain spectral data; S4, taking N points on each to-be-detected film, measuring the thickness of the to-be-detected film, and taking a mean value as the thickness of the to-be-detected film; and S5, calculating, analyzing and recombining the frequency domain spectral data of each pixel point of the measurement film, and converting the frequency domain spectral data intogray values of the corresponding pixel points, so as to form a visual image reflecting the spatial distribution condition of the biomacromolecule content on the film. The method has the advantages ofno need of antibodies, simple operation, fast and accurate detection speed, time saving, labor cost saving, and realization of label-free detection.

Description

technical field [0001] The invention belongs to the field of biological macromolecule detection, and relates to a relative quantitative detection method of biological macromolecule content based on terahertz imaging. Background technique [0002] The commonly used biomolecular detection technology is immunostaining (Western Blot), which is an experimental method commonly used in molecular biology, biochemistry and immunogenetics. The basic principle is to stain cells or biological tissue samples processed by gel electrophoresis with specific antibodies. Information on the expression of a specific protein in the cell or tissue being analyzed is obtained by analyzing the location and depth of staining. [0003] The antigen-antibody reaction brings better specificity, but at the same time it also brings more complicated operations and the attendant material, time and labor costs. Moreover, for some proteins for which high-quality antibodies cannot be obtained, the accuracy of...

AI Technical Summary

Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a method for relative quantitative detection of biomacromolecules based on terahertz imaging, which can solve the defects of existing methods for quantitative and qualitative analysis of proteins based on immunological techniques, such as antibodies (antibody efficacy) High price, high sensitivity is the decisive factor for the success of the experiment), and the material, time and labor costs caused by complex operation

Images