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Non-enzymatic biosensor based on carbon material/boron-doped diamond composite electrode as well as preparation method and application of non-enzymatic biosensor

A boron-doped diamond and biosensor technology, applied in the field of non-enzyme biosensor preparation, can solve the problems of poor repeatability of sample preparation, high preparation cost, poor combination of nano-sensitive materials and carriers, etc.

Active Publication Date: 2020-08-21
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current preparation methods of metal nanomaterials modified composite carbon material electrodes, such as hydrothermal method, electrodeposition method, etc., often require secondary loading, resulting in poor bonding between the nano-sensitive material and the carrier in the composite electrode, and poor repeatability of sample preparation. Higher preparation cost

Method used

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  • Non-enzymatic biosensor based on carbon material/boron-doped diamond composite electrode as well as preparation method and application of non-enzymatic biosensor
  • Non-enzymatic biosensor based on carbon material/boron-doped diamond composite electrode as well as preparation method and application of non-enzymatic biosensor
  • Non-enzymatic biosensor based on carbon material/boron-doped diamond composite electrode as well as preparation method and application of non-enzymatic biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Step 1, silicon substrate pretreatment. First place the silicon substrate in an acetone solution for 10 minutes of ultrasonic treatment to remove surface oil; then place it in nano-diamond seed crystal suspension for 30 minutes to increase the nucleation rate of diamond growth; finally place it in anhydrous Sonicate in ethanol for 5 minutes;

[0040] Step 2, preparation of boron-doped diamond film. Hot wire chemical vapor deposition of boron-doped diamond is adopted. During the deposition process, the reaction gas is fed into and includes hydrogen, methane, and borane. The hydrogen flow rate is 98 sccm, the methane flow rate is 2 sccm, and the borane flow rate is 0.4 sccm. The filament temperature is 2500-2700°C, the deposition temperature is 800-850°C, the deposition pressure is 3kPa, and the deposition time is 12h.

[0041] Step 3, preparation of the nickel catalyst layer. A nickel layer is deposited on the surface of boron-doped diamond by magnetron sputtering. In...

Embodiment 2

[0045] Step 1, silicon substrate pretreatment. First place the silicon substrate in an acetone solution for 10 minutes of ultrasonic treatment to remove surface oil; then place it in nano-diamond seed crystal suspension for 30 minutes to increase the nucleation rate of diamond growth; finally place it in anhydrous Sonicate in ethanol for 5 minutes;

[0046] Step 2, preparation of boron-doped diamond film. Hot wire chemical vapor deposition of boron-doped diamond is adopted. During the deposition process, the reaction gas is fed into and includes hydrogen, methane, and borane. The hydrogen flow rate is 98 sccm, the methane flow rate is 2 sccm, and the borane flow rate is 0.4 sccm. The filament temperature is 2500-2700°C, the deposition temperature is 800-850°C, the deposition pressure is 3kPa, and the deposition time is 12h.

[0047] Step 3, preparation of the nickel catalyst layer. A nickel layer is deposited on the surface of boron-doped diamond by magnetron sputtering. In...

Embodiment 3

[0051] Step 1, silicon substrate pretreatment. First place the silicon substrate in an acetone solution for 10 minutes of ultrasonic treatment to remove surface oil; then place it in nano-diamond seed crystal suspension for 30 minutes to increase the nucleation rate of diamond growth; finally place it in anhydrous Sonicate in ethanol for 5 minutes;

[0052] Step 2, preparation of boron-doped diamond film. Hot wire chemical vapor deposition of boron-doped diamond is adopted. During the deposition process, the reaction gas is fed into and includes hydrogen, methane, and borane. The hydrogen flow rate is 98 sccm, the methane flow rate is 2 sccm, and the borane flow rate is 0.4 sccm. The filament temperature is 2500-2700°C, the deposition temperature is 800-850°C, the deposition pressure is 3kPa, and the deposition time is 12h.

[0053] Step 3, preparation of the nickel catalyst layer. A nickel layer is deposited on the surface of boron-doped diamond by magnetron sputtering. In...

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Abstract

The invention relates to a non-enzymatic biosensor based on a carbon material / boron-doped diamond composite electrode as well as a preparation method and application of the non-enzymatic biosensor. Aworking electrode of the non-enzyme biosensor is a carbon material / boron-doped diamond composite electrode; the carbon material / boron-doped diamond composite electrode comprises a substrate, a boron-doped diamond layer arranged on the surface of the substrate and a carbon material arranged on the surface of the boron-doped diamond layer, the carbon material is selected from at least one of microcrystalline graphite, carbon nanotubes, carbon nanofibers and graphene, the carbon nanotubes are carbon nanotubes growing at the bottom end, the boron-doped diamond film is of a surface porous structure, and meanwhile the surface of the boron-doped diamond film is further modified with nickel nanoparticles. By combining chemical vapor deposition, magnetron sputtering and annealing, nickel catalyticpreparation of different composite material electrodes is realized. The prepared composite carbon material electrode has the characteristics of high sensitivity, high stability, high resolution and high selectivity, and can be widely applied to the construction of glucose sensors.

Description

technical field [0001] The invention relates to a non-enzyme biosensor based on a carbon material / boron-doped diamond composite electrode, a preparation method and application thereof, and belongs to the technical field of non-enzyme biosensor preparation. Background technique [0002] A biosensor (biosensor) is a device or device that organically combines biologically active materials (enzymes, proteins, DNA, antibodies, antigens, biofilms, etc.) It is also a rapid and trace analysis method at the molecular level of substances. According to the definition, the structure (composition) of a biosensor includes two parts: 1. Bioactive materials (also called biosensitive membranes, molecular recognition elements). 2. Physical transducers (also called sensors). Among them, this patent relates to the sensor part, and its function is to convert various biological, chemical and physical information into electrical signals. The information generated by the biological reaction proc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327C23C16/27C23C16/02C23C14/16C23C14/35C23C16/26C23C16/44C23C28/00
CPCG01N27/327C23C16/271C23C16/278C23C16/02C23C14/165C23C14/35C23C16/26C23C16/44C23C28/30
Inventor 魏秋平马莉周科朝曾思超朱睿童杨万林
Owner CENT SOUTH UNIV
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