Application of prussian blue nanoparticles in preparation of drugs for preventing, delaying or treating nervous system degenerative diseases
A nervous system degenerative and Prussian blue technology, applied in the field of biomedicine, can solve the problems of no relevant reports on the study of nervous system degenerative diseases, achieve the effects of improving learning and memory, increasing the proportion of living cells, and facilitating mass production
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Embodiment 1
[0048] In this example, a Prussian blue nanoparticle without functional modification was prepared. The preparation method includes the following steps:
[0049] (1) Dissolve 0.01 mM potassium ferrocyanide and 0.001 mM m PEG-COOH (MW5000) in 5 mL deionized water, mix well to obtain a clear solution A; dissolve 0.01 mM ferric chloride in 5 mL deionized water, Fully dissolve to obtain clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;
[0050] (2) After the reaction system is lowered to 25°C, react for 1 hour, centrifuge and wash to obtain Prussian blue nanoparticles without functional modification.
[0051] The prepared Prussian blue nanoparticles were characterized by particle size and potential. The result was that the dynamic light scattering particle size was 80nm and the surface potential was -32mV.
Embodiment 2
[0053] In this example, a single-targeted modified Prussian blue nanoparticle was prepared. The preparation method includes the following steps:
[0054] (1) Dissolve 0.01 mM potassium ferrocyanide and 0.001 mM m PEG-COOH (MW5000) in 5 mL deionized water, mix well to obtain a clear solution A; dissolve 0.01 mM ferric chloride in 5 mL deionized water, Fully dissolve to obtain clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;
[0055] (2) After the reaction system is lowered to 25°C, react for 1 hour, centrifuge and wash, to obtain Prussian blue nanoparticles without functional modification;
[0056] (3) Dissolve the obtained Prussian blue nanoparticles, 0.01 mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in 10 mL of deionized water, and react at 25°C for 15 minutes to obtain a solution D;
[0057] (4) Add 0.01mM N-hydroxysuccinimide and 0.4μM Congo red to solution D, react at 25°C for 24h, centrifuge and wash, and the resulting pre...
Embodiment 3
[0060] In this example, a dual-targeted modified Prussian blue nanoparticle was prepared. The preparation method includes the following steps:
[0061] (1) Dissolve 0.01 mM potassium ferrocyanide and 0.001 mM m PEG-COOH (MW5000) in 5 mL deionized water, mix well to obtain a clear solution A; dissolve 0.01 mM ferric chloride in 5 mL deionized water, Fully dissolve to obtain clear solution B; add solution B dropwise to solution A, and react at 60°C for 1 hour;
[0062] (2) After the reaction system is lowered to 25°C, react for 1 hour, centrifuge and wash, to obtain Prussian blue nanoparticles without functional modification;
[0063] (3) Dissolve the obtained Prussian blue nanoparticles, 0.01 mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in 10 mL of deionized water, and react at 25°C for 10 minutes to obtain a solution D;
[0064] (4) Add 0.01mM N-hydroxysuccinimide, 0.005mg transferrin, and 0.5μM Congo red to solution D, react at 25°C for 24h, centrifuge and wash, a...
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