One-tube nested real-time quantitative PCR detection test kit for detecting alveolar echinocococosis and cystic echinococcosis by micro-sample typing
A technology for Echinococcus multilocularis and Echinococcus granulosus, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., and can solve the problem of low copy number, aerosol contamination, nested PCR Difficult to clinical diagnosis and other problems, to achieve the effect of high sensitivity, high accuracy and easy operation
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[0038] The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Of course, the described embodiments are only some, not all, embodiments of the present invention, and do not imply any limitation to the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0039] In this example, the present invention finds that CBLO020001206 can be used for the in vitro diagnosis of Echinococcus multilocularis and Echinococcus granulosus infection based on the sequencing of free DNA in the plasma of echinococcosis patients, and designs and screens out Echinococcus multilocularis and Echinococcus granulosus infection The outer primer pair, inner primer pair, inner quality control gene primer pair and probe were designed for the specific...
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