One-tube nested real-time quantitative PCR detection kit for micro-sample typing and detection of Echinococcus multilocularis and Echinococcus granulosus
A technology for Echinococcus multilocularis and Echinococcus granulosus, applied in biochemical equipment and methods, microbe determination/inspection, DNA/RNA fragments, etc., can solve low copy number, aerosol pollution, nested PCR Difficult clinical diagnosis and other problems, to achieve the effect of high sensitivity, high accuracy, and easy operation
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[0037] The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Of course, the described embodiments are only some, not all, embodiments of the present invention, and do not imply any limitation to the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0038] In this example, the present invention finds that CBLO020001206 can be used for the in vitro diagnosis of Echinococcus multilocularis and Echinococcus granulosus infection based on the sequencing of free DNA in the plasma of echinococcosis patients, and designs and screens out Echinococcus multilocularis and Echinococcus granulosus infection The outer primer pair, inner primer pair, inner quality control gene primer pair and probe were designed for the specific...
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