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Application of esophageal cancer related antibody protein combination in colloidal gold test strip

A technology for colloidal gold and esophageal cancer, applied in the fields of molecular biology and oncology, can solve the problem of low expression, achieve the effects of less trauma, reduced incidence, and high specificity and sensitivity

Active Publication Date: 2020-08-07
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ALDH3A1 is a subtype of the aldehyde dehydrogenase family, which exists in the stomach, esophagus and lung tissues, but is less expressed in the liver and kidney

Method used

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  • Application of esophageal cancer related antibody protein combination in colloidal gold test strip
  • Application of esophageal cancer related antibody protein combination in colloidal gold test strip
  • Application of esophageal cancer related antibody protein combination in colloidal gold test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Application of tumor-associated antigens in the detection of esophageal cancer

[0043] Through the detection and analysis of specific antigens in the serum of 60 patients with early esophageal cancer and 60 normal people from high-incidence areas, it was found that the expression of five antigens, FAM84A, USP15, FAM212B, ALDH3A1, and RAB33B, was significantly higher in the serum of early esophageal cancer in normal population.

[0044] 1. Experimental samples

[0045] Sera from 60 patients with early esophageal cancer from Anyang Cancer Hospital and 60 normal controls from Zhengtun Village, Mengzhuang Town, Xinxiang City were selected.

[0046] The sera of 60 patients with early esophageal cancer were obtained from patients with early (stage 0+stage I) esophageal cancer confirmed by histopathology, and none of them received radiotherapy or chemotherapy; none of the 60 normal subjects had any tumor-related medical history. Among the 60 patients with esophage...

Embodiment 25

[0069] Application of Example 25 Proteins in the Preparation of Colloidal Gold Test Strips for Esophageal Cancer Asymptomatic High-Risk Group Screening and Early Detection

[0070]This application prepares a colloidal gold test strip for the screening and early detection of asymptomatic high-risk groups of esophageal cancer, which includes a PVC bottom plate, and the bottom plate is provided with a sample pad and a colloidal gold-marked binding pad sequentially connected from one end thereof , nitrocellulose membrane and absorbent pad; the colloidal gold-labeled binding pad is coated with colloidal gold-labeled FAM84A monoclonal antibody, USP15 monoclonal antibody, FAM212B monoclonal antibody, ALDH3A1 monoclonal antibody, RAB33B monoclonal antibody A mixture of antibodies; the nitrocellulose membrane is provided with a control line and five detection lines, and the five detection lines are respectively immobilized with a rabbit anti-human FAM84A polyclonal antibody, a rabbit an...

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Abstract

The invention belongs to the field of molecular biology and oncology, and particularly discloses the application of a combination of FAM84A protein, USP15 protein, FAM212B protein, ALDH3A1 protein andRAB33B protein in an esophageal cancer detection colloidal gold test strip or a kit. The present invention also discloses the colloidal gold test strip for screening and early discovering the asymptomatic high-risk groups. The colloidal gold test strip comprises a bottom plate, and a sample pad, a colloidal gold labeled combination pad, a nitrocellulose membrane and a water absorption pad which are sequentially connected from one end of the bottom plate are arranged on the bottom plate. The colloidal gold test strip provided by the invention improves the sensitivity and specificity of esophageal cancer symptomless high-risk group screening and early esophageal cancer, and when the five tumor markers are combined at the same time, the detection effect reaches the best (the sensitivity is 92.3%, and the specificity is 98.3%).

Description

technical field [0001] The invention belongs to the fields of molecular biology and oncology, and specifically relates to the application of esophageal cancer-related antibody protein combinations in the screening of asymptomatic high-risk groups and early detection of colloidal gold test strips. Background technique [0002] Esophageal cancer is the eighth most common malignancy and the fifth most common cause of cancer death in the world. Esophageal cancer has obvious differences in geographical distribution. More than half of the new cases of esophageal cancer in the world occur in my country every year, especially in the Taihang Mountain area at the junction of Henan, Hebei, and Shanxi provinces. The incidence and mortality rate rank first in the world. . The high morbidity and mortality of esophageal cancer are also one of the important reasons why residents in these areas become poor and return to poverty due to illness. The prognosis of patients with advanced esophag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/574G01N33/558
CPCG01N33/558G01N33/57407G01N33/57488G01N33/577G01N2800/60
Inventor 王立东宋昕王伟赵学科李贝韩雪娜范宗民王苒杨苗苗王盼盼陈瑶
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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