Kit for detecting human herpes virus infection and detection method thereof
A human herpes virus and herpes virus technology, applied in the field of biomedicine, can solve the problem of long incubation time, and achieve the effects of long incubation time, rapid monitoring, and prevention of antibiotic abuse
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[0059] The technical solutions of the present invention will be described in detail below in conjunction with the drawings and embodiments, but the present invention is not limited to the scope of the embodiments.
[0060] For the experimental methods that do not specify specific conditions in the following examples, select according to conventional methods and conditions, or according to the product instructions. The reagents and raw materials used in the present invention are all commercially available.
[0061] 1. The experimental steps of nucleic acid extraction are as follows:
[0062] 1. Centrifuge the clinical whole blood sample (10mL) at 2950rpm for 15min, take 2mL of the upper layer of plasma into the reagent compartment hole 1 of the automatic extractor, and store the remaining samples in a 15mL centrifuge tube at -80°C.
[0063] 2. Add 200 μl Proteinase K, 30 μl magnetic beads and 1 μl Carrier RNA to well 1 respectively.
[0064] 3. Add 1mL buffer GDF to well 2. ...
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