Grape fermentation puree as well as preparation method and application thereof
A technology for fermenting puree and grapes, which is applied in the field of fermented products, can solve the problems of consuming medicinal materials, technology cannot be popularized, and breakthroughs are difficult to achieve, so as to avoid the loss of active ingredients, inhibit tyrosinase activity, and maintain natural activity.
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Embodiment 1
[0046] The concentration of fermentation bacteria in the expansion medium was 10 5 -10 8Inoculate 10mL of CFU / ml fermented bacteria liquid into 100g of grape pulp and 1000mL of water, and incubate in a 40°C incubator for 12 hours, then autoclave the obtained fermented liquid at 121°C for 15min. Inactivate the bacteria, centrifuge the sterilized fermented liquid for 20 min under the conditions of 4500 r / min and a centrifugal radius of 9 cm, and collect the supernatant, which is the grape fermentation puree cosmetic provided by the present invention.
[0047] The appearance of the grape fermented puree cosmetic prepared in this example is a viscous liquid, and the color is colorless, transparent to brown. The pH value is 4.5-6.8, the viscosity is 100-800cP, the soluble solid content is 1.0-5.0%, the total number of colonies is less than 50CFU / ml, and no pathogenic bacteria are detected. It meets the quality requirements of cosmetics.
[0048] Component analysis of the grape f...
Embodiment 2
[0107]The concentration of fermentation bacteria in the expansion culture medium was 10 5 -10 8 Inoculate 40 mL of CFU / ml fermented bacteria liquid into 100 g of grape pulp and 1500 mL of water, and incubate in a 30°C incubator for 30 hours, then autoclave the resulting fermented liquid at 121°C for 15 minutes. Inactivate the bacteria, centrifuge the sterilized fermented liquid for 30 min under the conditions of 4000 r / min and a centrifugal radius of 12 cm, and collect the supernatant, which is the grape fermentation puree cosmetic provided by the present invention.
[0108] The appearance of the grape fermented puree cosmetic prepared in this example is a viscous liquid, and the color is colorless, transparent to brown. The pH value is 4.5-6.8, the viscosity is 100-800cP, the soluble solid content is 1.0-5.0%, the total number of colonies is less than 50CFU / ml, and no pathogenic bacteria are detected. It meets the quality requirements of cosmetics.
Embodiment 3
[0110] The concentration of fermentation bacteria in the expansion culture medium was 10 5 -10 8 Inoculate 20mL of CFU / ml fermentation broth into 400g of grape pulp and 800mL of water, and incubate in a 45°C incubator for 8 hours, then autoclave the resulting fermentation broth at 121°C for 15min. Inactivate the bacteria, centrifuge the sterilized fermented liquid for 15 min under the conditions of 12000 r / min and a centrifugal radius of 10 cm, and collect the supernatant, which is the grape fermentation puree cosmetic provided by the present invention.
[0111] The appearance of the grape fermented puree cosmetic prepared in this example is a viscous liquid, and the color is colorless, transparent to brown. The pH value is 4.5-6.8, the viscosity is 100-800cP, the soluble solid content is 1.0-5.0%, the total number of colonies is less than 50CFU / ml, and no pathogenic bacteria are detected. It meets the quality requirements of cosmetics.
[0112] The component analysis metho...
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