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Application of 3,5-dichloroanthranilic acid to induce Arabidopsis resistance to Botrytis cinerea and its method

An anthranilic acid and Arabidopsis technology, which is applied in the field of compound inducing plant resistance, can solve the problems of poor water solubility of the inducer, high cost of Botrytis cinerea, and pollute the environment, so as to achieve no secondary pollution, improve resistance, good water solubility

Active Publication Date: 2022-05-27
TAIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide the application of 3,5-dichloroanthranilic acid to induce the resistance of Arabidopsis to Botrytis in order to solve the problems of high cost, environmental pollution and poor water solubility of the inducer in preventing and treating Botrytis thaliana at present. and its methods

Method used

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  • Application of 3,5-dichloroanthranilic acid to induce Arabidopsis resistance to Botrytis cinerea and its method
  • Application of 3,5-dichloroanthranilic acid to induce Arabidopsis resistance to Botrytis cinerea and its method
  • Application of 3,5-dichloroanthranilic acid to induce Arabidopsis resistance to Botrytis cinerea and its method

Examples

Experimental program
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Effect test

Embodiment 1

[0037]Example 1: Cultivation of Arabidopsis thaliana plants

[0038] The seeds of wild-type Arabidopsis (Col-0) were suspended in 0.1% agarose solution, vernalized at low temperature at 4 °C for 2-4 days, sown in mixed nutrient soils (vermiculite: grass and wood ash: perlite = 6:2:1), fertilized, diluted with water according to mass volume ratio l: 1000, coated for 2-3 days to facilitate seedling emergence. The incubation temperature is maintained at 22-24 °C, the relative humidity is 80%, and the light intensity is about 85 μmol·s -1 ·m -2 , the light time is 16 hours. Other water, pest and cultivation management are routine. 4-week-old Arabidopsis plants were used for experimental treatment.

Embodiment 2

[0039] Example II: Treatment of 3,5-dichloro-o-aminobenzoic acid

[0040] Configure 10 μM of aqueous 3,5-dichloro-o-aminobenzoic acid. Irrigated Arabidopsis plants were treated with an aqueous solution of 10 μM of 3,5-dichloro-o-aminobenzoic acid. Irrigated Arabidopsis plants with the same volume of water as a control group, after 2 days, inoculation of gray molds was carried out.

Embodiment 3

[0041] Example 3: Arabidopsis thaliana treated from Example 2 is inoculated with gray mold

[0042] With 2× V8 solid medium (36% V8 juice, 0.2% CaCO 3 , 2% agar) to cultivate gray mold at a culture temperature of 20-25 °C. After the dish is full of spores, place the hyphal blocks into a resuspension (maltose 4%, tryptone 1%) and vortex vigorously to release the spores, filter out the spores with gauze, and adjust their spore concentrations to 2×10 with a hemocytometer plate 5 Spores / mL. During inoculation, spray the spore suspension evenly onto the inoculated seedlings until most of the leaves are covered with tiny droplets. Inoculate seedlings are placed at 24 °C with a photoperiod of 12 h light / 12 h dark and a light intensity of 200 μE·m -2 S -1 After 4 days of high moisturizing, the onset of the film was observed. The same volume of resuspension was sprayed on Arabidopsis control plants as a control (MOCK).

[0043] If attached Figure 1 As shown, Example THREE pre-treated with ...

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Abstract

The invention discloses the application and method of 3,5-dichloro-anthranilic acid inducing resistance to Botrytis cinerea in Arabidopsis, belonging to the field of compound-induced plant resistance; in the present invention, 3,5-dichloro-ortho An aqueous solution of anthranilic acid was used to inoculate Botrytis cinerea two days after root irrigation or page spraying of four-week-old Arabidopsis thaliana. The results showed that Arabidopsis seedlings treated with 3,5-dichloroanthranilic acid showed The resistance to gray mold is obviously increased; 3,5-dichloroanthranilic acid induces Arabidopsis to produce resistance to gray mold, and has the advantages of good water solubility, small amount of use, safety, green, cheap and easy to obtain.

Description

Technical field [0001] The present invention belongs to the field of compound-induced plant resistance, relates to the application of a compound in plant-induced resistance and method thereof, specifically to the application of 3,5-dichloro-o-aminobenzoic acid to induce arabidopsis resistance to gray mold and method thereof. Background [0002] 3,5-Dichloroanthranilic Acid (3,5-Dichloroanthranilic Acid, DCA), alias 2-amino-3,5-dichlorobenzoic acid, is a white crystal powder, low toxicity, commonly used as an intermediate in medicine and fragrance. The chemical formula is C7H5Cl2NO2, the molecular weight is 206.026, the density is 1.607g / cm3, the melting point is 227-230 °C (lit.), the boiling point is 344.4ºCat760mmHg, and the CAS registration number is 2789-92-6. [0003] When people studied the growth and development of plants in depth, plants such as corn, tomatoes, peas, rice, barley, morning glory and snapdragon were studied as model gene systems, but the results were not sa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/44A01P21/00A01P3/00A01G7/06
CPCA01N37/44A01G7/06
Inventor 张译匀陆玲悦张慧娟
Owner TAIZHOU UNIV
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