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A kind of cryopreservation method of Moringa pollen

A technology for ultra-low temperature preservation and pollen, which is applied in the field of ultra-low temperature preservation of Moringa oleifera pollen, to achieve the effects of solving the problem of flowering failure, increasing production and reducing waste

Inactive Publication Date: 2021-09-28
YUNNAN INST OF TROPICAL CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Have not yet seen how to use ultra-low temperature technology to preserve Moringa pollen and extend the pollen viability of Moringa to about 2-3 months. Problems urgently needed to be solved by those skilled in the art

Method used

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  • A kind of cryopreservation method of Moringa pollen
  • A kind of cryopreservation method of Moringa pollen
  • A kind of cryopreservation method of Moringa pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Choose to collect Moringa oleifera at 9:30 am on a sunny day when the flowers have just opened and the anthers have just opened and started to loose powder. Bring the collected flowers back to the laboratory, spread the pollen evenly on the treated medium, and culture them in an artificial climate box for 4 hours. The temperature and humidity are controlled at RH50%, 30°C and RH35%, 37°C, 4h. Afterwards, the pollen germination was observed under an Olympus 10×10 magnification microscope, and the microscopic examination counted. Each sample was repeated 3 times, and 3 fields of view were observed for each repetition. The measurement results are shown in Table 1. The best condition for in vitro germination of Moringa oleifera pollen is high temperature drying.

[0025] Table 1 Effects of pollen germination culture conditions on pollen viability in vitro

[0026]

Embodiment 2

[0028] Collect Moringa oleifera just opened in the afternoon on a sunny day, and the anthers just opened and start to loose powder, bring the flowers back to the laboratory to do 4 groups of experiments respectively, the experimental conditions are:

[0029] (1) Room temperature (35°C) without pre-freezing, store at -80°C for 24h

[0030] (2) Pre-freeze at -20°C for 1 hour, store in liquid nitrogen for 24 hours

[0031] (3) Pre-freeze at -20°C for 1 hour, store at -80°C for 24 hours

[0032] (4) Pre-freeze at -20°C for 1 hour, then pre-freeze at -80°C for 1 hour, and store in liquid nitrogen for 24 hours;

[0033] Then the Moringa oleifera flowers treated under the above-mentioned 4 conditions are respectively thawed for 10-20min in the artificial climate box of 20% and 15°C in temperature and humidity, and then placed in the artificial climate box of 30% and 35°C in temperature and humidity respectively. After recovery for 40 minutes, the pollen germination rate is shown in...

Embodiment 3

[0037] Collect Moringa oleifera just opened in the afternoon on a sunny day, and the anthers just opened and started to shed powder. The flowers were brought back to the laboratory and pre-frozen at -20°C for 1 hour, then at -80°C for 1 hour, and then stored in liquid nitrogen for 24 hours. , 4 groups of experiments were carried out after the flowers were taken out, and the experimental conditions were

[0038] (1) Warm water bath at 38°C for 3-5min

[0039] (2) Thaw at RH20% and 15°C for 20 minutes, then resuscitate at RH50% and 35°C for 40 minutes

[0040] (3) Thaw at RH20% and 15°C for 20 minutes, then resuscitate at RH30% and 35°C for 40 minutes

[0041] (4) Thaw at RH20% and 15°C for 20 minutes, then recover at RH30% and 35°C for 3 hours

[0042] After treatment according to the above four thawing methods, the pollen germination rate is shown in Table 3. The results show that Moringa oleifera pollen is stored at ultra-low temperature, and then placed in an artificial cl...

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Abstract

The invention discloses a method for ultra-low temperature preservation of Moringa oleifera pollen, which comprises the following steps: (1) selecting flowers with pollen from 9 am to 11 am; (2) placing the flowers in step (1) in a sealed bag , dried, sealed, pre-frozen, and then stored at (-80)°C-(-200)°C for future use; (3) thawing the flowers obtained in step (2) to obtain the Moringa oleifera pollen. The cryopreservation method among the present invention can preserve complete Moringa flower, and the maintenance time of pollen vigor can be extended to 2-3 months by normal 1-2 days, prolongs the life-span of pollen; Among the present invention The thawing method can avoid secondary freezing in the cells during the thawing process, and prevent the osmotic shock of water from damaging the cell membrane system during the thawing process, thereby effectively ensuring the effect of ultra-low temperature freezing of pollen.

Description

technical field [0001] The invention relates to the technical field of preservation of plant pollen, in particular to a method for ultra-low temperature preservation of Moringa oleifera pollen. Background technique [0002] Moringa originated in the arid or semi-arid regions of India and Africa. With its strong ecological adaptability, rich nutritional value, and unique biological activity, it has become a tree species with great development potential. Moringa seed oil is an excellent raw material for high-grade cooking oil, cosmetics, spices and preservatives. Moringa seed oil can also be used as a lubricant for aerospace and precision machinery. In addition, Moringa seed kernels are rich in active coagulation components, which have the effect of purifying water, so Moringa is also known as "plant gold". [0003] Pollen is one of the forms of plant germplasm, which contains all gene types of the species and has extremely rich genetic information. It is an important materi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 吕亚李海泉张祖兵任保兰
Owner YUNNAN INST OF TROPICAL CROPS
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