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Method for inducing initial bud by using sterile podocarpus macrophyllus explant

A technology of explants and Podocarpus, applied in the field of plant tissue culture, can solve the problems of easy variation in offspring, poor growth consistency, and inability to make full use of high-quality provenance, so as to achieve vigorous vitality, vigorous vegetative growth, and good economic benefits. and the effect of social benefits

Inactive Publication Date: 2020-08-07
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at technical problems such as sowing and cutting that the current main propagation methods of Podocarpus pine are unable to make full use of high-quality provenance, high-quality individual plants, high genetic gain, easy variation in offspring, poor growth consistency, etc., and provides a method of using The method of inducing initial buds from Podocarpus aseptic explants lays the foundation for the large-scale promotion of high-quality provenance and high-quality individual plants, achieving high genetic gain and ensuring relatively consistent growth of offspring

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The annual Podocarpus seedlings were used as the rootstock, and the young shoots of the excellent plants of Podocarpus from Guangxi were selected as the scion. After the scion was cut from an adult high-quality Podocarpus pine tree, it was wrapped with a wet towel, put into an incubator equipped with ice packs, brought back to refrigerate at 4°C, and grafted by conventional methods within 24 hours. After the grafting survived, the main shoots of the grafted seedlings were knocked out to promote the growth of buds, and when the sprouted strips reached 3.0-5.0 cm long, they were cut and used as explants for subsequent use.

[0020] After the explant was rinsed with sterile water, it was placed in a volume concentration of 75% alcohol and soaked for 3 minutes, taken out and placed in a solution of 10% volume concentration of hydrogen peroxide (adding 5 drops of Tween) and stirred for 20 minutes. , take out the explants and soak them in a broad-spectrum fungicide with a vol...

Embodiment 2

[0024] The annual Podocarpus seedlings were used as the rootstock, and the young shoots of the excellent plants of Podocarpus from Guangxi were selected as the scion. After the scion was cut from an adult high-quality Podocarpus pine tree, it was wrapped with a wet towel, put into an incubator equipped with ice packs, brought back to refrigerate at 4°C, and grafted by conventional methods within 24 hours. After the grafting survived, the main shoots of the grafted seedlings were knocked out to promote the growth of buds. When the sprouting strips reached 3.0-5.0 cm in length, the conventional method was used to spray 0.1% carbendazim solution with a volume concentration. After 1-2 days, the harvested plants were vigorous in fine weather. , and the long sprout strips with a length of 3.0-5.0 cm are used as explants for subsequent use.

[0025] After the explant was rinsed with sterile water, it was placed in a volume concentration of 75% alcohol and soaked for 4 minutes, taken ...

Embodiment 3

[0029] The annual Podocarpus seedlings were used as the rootstock, and the young shoots of the excellent plants of Podocarpus from Guangxi were selected as the scion. After the scion was cut from an adult high-quality Podocarpus pine tree, it was wrapped with a wet towel, put into an incubator with ice packs, brought back to refrigerate at 4°C, and grafted by conventional methods within 24 hours. After the grafting survived, the main shoots of the grafted seedlings were knocked out to promote the growth of buds. When the sprouting strips reached 3.0-5.0 cm in length, the conventional method was used to spray 0.1% thiophanate-methyl solution in a volume concentration, and the vigor was harvested in fine weather 1-2 days later. Exuberant, long 3.0-5.0cm long sprout strips are used as explants for subsequent use.

[0030]After the explant was rinsed with sterile water, it was placed in a volume concentration of 75% alcohol and soaked for 5 minutes, taken out and placed in a solut...

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Abstract

The invention discloses a method for inducing an initial bud by using a sterile podocarpus macrophyllus explant. The method comprises the following steps of taking a sprouting branch of a grafted seedling of podocarpus macrophyllus as an explant, performing disinfection, inoculating the explant into an induction culture medium, and performing initial bud induction culture until sprouting to obtainthe initial bud of podocarpus macrophyllus. According to the method, sprouting branches of grafted seedlings of podocarpus macrophyllus serve as explants, the juvenilization degree is high, the effectiveness of the explants is greatly improved, the initial bud occurrence rate is high, bud seedling elongation is high, vitality is vigorous, consistency is high, and a foundation is laid for large-scale popularization of high-quality seed source and excellent single plants, obtaining of relatively high genetic gain and guaranteeing of relative consistency of offspring growth, and relatively higheconomic benefits and social benefits are realized.

Description

technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for inducing initial buds by utilizing Podocarpus aseptic explants. Background technique [0002] Podocarpus macrophyllus (Scientific name: Podocarpus macrophyllus (Thunb.) D.Don), also known as soil fir, Podocarpus macrophyllus, Podocarpus family, Podocarpus is an evergreen coniferous tree, up to 20m, diameter at breast height of 60cm, flowering period from April to May, seeds 8-9 month ripe. Podocarpus is produced in Jiangsu, Zhejiang, Fujian, Anhui, Jiangxi, Hunan, Sichuan, Yunnan, Guizhou, Guangxi, Guangdong and other provinces and regions in China, and it is also distributed in Japan. The material is fine and uniform, easy to process, and can be used as furniture, utensils, stationery and agricultural tools. [0003] Podocarpus is a coniferous tree species, the plant has a variety of medicinal value, such as leaves can be used to stop bleeding, treatment of bru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 莫昭展卢娟林桂安苏建睦
Owner YULIN NORMAL UNIVERSITY
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