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Autologous and homogeneous adipose-derived mesenchymal stem cell composition for curing tendon or ligament injury and preparation method thereof

A stem cell and adipose-derived technology, applied in drug combinations, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems such as unmentioned methods and optimal culture conditions, and achieve the effect of improving tendon or ligament diseases

Pending Publication Date: 2020-08-04
ANTEROGEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the methods and optimal culture conditions for using adipose-derived mesenchymal stem cells to effectively treat tendon-related diseases are not mentioned in the above-mentioned documents

Method used

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  • Autologous and homogeneous adipose-derived mesenchymal stem cell composition for curing tendon or ligament injury and preparation method thereof
  • Autologous and homogeneous adipose-derived mesenchymal stem cell composition for curing tendon or ligament injury and preparation method thereof
  • Autologous and homogeneous adipose-derived mesenchymal stem cell composition for curing tendon or ligament injury and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0064] The present invention relates to a method for preparing a composition comprising adipose tissue-derived mesenchymal stem cells for improving or treating tendon or ligament diseases, which includes: step (a), isolating the mesenchymal stem cells-vascular part from adipose tissue and Cultivate in stromal medium; step (b), in a medium containing selected from fibroblast growth factor (Fibroblast GrowthFactor, FGF), epidermal growth factor (Epidermal Growth Factor, EGF), transforming growth factor β-1 ((transforming growth factor beta -1, TGFβ-1), platelet-derived growth factor (platelet-derived growth factor, PDGF), vascular endothelial growth factor (vascular endothelial growth factor, VEGF), hepatocyte growth factor (hepatocyte growth factor, HGF) and insulin-like growth factor (insulin-like growth factor, IGF-1) in the culture medium of more than one growth factor in the group consisting of culturing the stromal-vascular part; and step (c), subculture the cultured mesenc...

Embodiment approach

[0094] Hereinafter, the present invention is illustrated in more detail by the following examples. However, this is only to help the understanding of the present invention, and is not intended to limit the scope of protection of the present invention.

Embodiment 1

[0096] Example 1: Culture method of human adipose-derived mesenchymal stem cells

[0097] Adipose-derived mesenchymal stem cells were isolated from adipose tissue obtained by liposuction (adipose tissue is usually, but not limited to, obtained by liposuction) in the following manner: To remove blood, the same volume of K-Lin Krebs-Ringer bicarbonate (KRB) solution washed the adipose tissue for 3 to 4 times. A collagenase solution of the same volume as that of adipose tissue was added and reacted in a water bath at a temperature of 37°C. This was transferred to a centrifuge tube, and centrifuged at 1200 rpm for 10 minutes at a temperature of 20°C. The fat layer as the supernatant was removed, and the collagenase solution as the lower layer was carefully separated to avoid shaking. After adding the matrix medium to suspend the mixture, it was centrifuged at 1200 rpm for 5 minutes at a temperature of 20°C. At this time, the mesenchymal stem cell-vascular fraction was precipita...

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Abstract

The invention relates to an autologous and homogeneous composition containing adipose-derived mesenchymal stem cells for improving or treating tendon or ligament diseases, a preparation method of thecomposition, and a treatment method of the tendon or ligament diseases. In particular, the adipose-derived mesenchymal stem cells are independently administrated or are administrated in combination with hydrogel; when the adipose-derived mesenchymal stem cells disclosed by the invention are administrated to achilles tendon diseases such as patellar tendon diseases, external epicondylitis, internalepicondylitis, plantar fasciitis, spinner muscle group diseases, tendon sheath synovitis, tendon degeneration, tendon inflammation, tenosynovitis, tendon injury, tendon relaxation and the like, and the ligament diseases such as cruciate ligament injury, ankle ligament injury, collateral ligament injury and the like, the collagen, extracellular matrix (ECM) protein, and various growth factors aresecreted, so that an effect of ameliorating or treating tendon or ligament diseases is exhibited.

Description

technical field [0001] The present invention relates to a composition comprising autologous or allogenic adipose-derived mesenchymal stem cells, a preparation method thereof, and a treatment method using the same, in particular to administration of adipose-derived mesenchymal stem cells alone or in combination with hydrogel. medicine, to improve or treat such as Achilles tendon disease, patellar tendon disease, lateral epicondylitis, medial epicondylitis, plantar fasciitis, rotator group disease, tenosynovitis, tendon degeneration, tendonitis, tenosynovitis A composition, a method for improving or treating tendon diseases such as cruciate ligament injury, ankle ligament injury, collateral ligament injury, etc., a method for improving or treating it, and a preparation method thereof. Background technique [0002] As a cell line with self-renewal ability, high viability, and differentiation potential to various cells, mesenchymal stem cells (Mesenchymal stem cells, MSCs) have ...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P19/04
CPCA61K35/28A61P19/04
Inventor 李晟丘金美馨
Owner ANTEROGEN
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