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Biocontrol bacterium BV03 and application and bacteria inoculant thereof, and preparation method of bacteria inoculant

A bio-control bacteria, BV03 technology, applied in the application and preparation of inoculants and inoculants, in the field of bio-control bacteria BV03, to achieve the effects of maximizing production benefits, convenient field application, and good control effects

Active Publication Date: 2020-07-31
ECOLOGY INST SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the hidden dangers of food safety caused by the excessive application of chemical pesticides existing in the prevention and treatment of Verticillium wilt, the invention provides a kind of biocontrol bacteria BV03, its application and its bacterial agent and its preparation method. Pepper and tomato) and / or Rosaceae plants (strawberry) Verticillium wilt have obvious biological control effect, especially can effectively sterilize pepper, tomato, strawberry seeds, prevent and control the reproduction of pathogenic bacteria of Verticillium wilt in fruits and vegetables, thereby saving a lot of cost, Maximize the benefits of fruit and vegetable production, and have great application value for the cultivation of economic plants

Method used

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  • Biocontrol bacterium BV03 and application and bacteria inoculant thereof, and preparation method of bacteria inoculant
  • Biocontrol bacterium BV03 and application and bacteria inoculant thereof, and preparation method of bacteria inoculant
  • Biocontrol bacterium BV03 and application and bacteria inoculant thereof, and preparation method of bacteria inoculant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 A kind of bacterial agent containing biocontrol bacteria BV03

[0024] A bacterial agent containing biocontrol bacteria BV03, the biocontrol bacteria BV03 is Bacillus Veles BV03, which has been preserved in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee, and the preservation address is Beichen, Chaoyang District, Beijing No. 3, No. 1 Yard, West Road, the preservation number is CGMCC NO.18936, the preservation date is November 11, 2019, the classification is named Bacillus velezensis, and the dosage form of the bacterial agent is a suspension concentrate;

[0025] The preparation method of bacterial agent comprises the steps:

[0026] (1) Inoculate the Bacillus Velez BV03 strain stored at -80°C into solid LB medium, culture it upside down at 30°C±1°C for 16 hours or overnight, pick a single colony, and inoculate it into liquid LB medium for 30 Cultivate to the logarithmic growth phase under the condi...

Embodiment 2

[0032] Seed soaking application of embodiment 2 bacterial agents in industrialized seedling cultivation

[0033] The test is divided into a biocontrol bacteria BV03 treatment group and a control group. The test method is to mix 0.5g of Verticillium dahliae thalline and 0.5g of Verticillium black and white thallus with each liter of sterilized nutrient soil, and mix well to obtain Cultivation medium, sow the seeds of the above treatment group and the control group according to the conventional method and routinely manage the fruit and vegetable plants, treat 33 plants in each group, repeat 3 times, observe and count the verticillium wilt of peppers caused by Verticillium dahliae / Verticillium black and white infection after 28 days The incidence and disease grade of Verticillium wilt of tomato, Verticillium wilt of strawberry and Verticillium wilt of strawberry. in,

[0034] Biocontrol bacteria BV03 treatment group: the concentration was 5.5×10 9 The bacterial agent of Example 1...

Embodiment 3

[0049] The root irrigation application of embodiment 3 bacterial agents

[0050] The test was designed according to the field test guidelines of the Institute for Drug Control of the Ministry of Agriculture. The test site was a facility field in Sunjia Village, Jinan City. The soil in the test site was yellow cinnamon soil with medium fertility and irrigation conditions. Peppers, tomatoes, and strawberries were planted in this area all year round. Verticillium wilt of pepper, Verticillium wilt of tomato and Verticillium wilt of strawberry caused by Verticillium dahliae and Verticillium black and white broke out during planting in 2018; the test crops were pepper "Zhongjiao No. 6" and tomato "Zhongyan 100"﹑strawberry "Xingdu No. 2"; the test time was May 2019, June 2019 and February 2019 respectively, and the amount and application method of the test fertilizer and field cultivation management were carried out according to the best local practices;

[0051] Five treatments were...

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Abstract

The invention relates to the field of microbial plant protection application technologies, in particular to a biocontrol bacterium BV03 and application and bacteria inoculant thereof, and a preparation method of the bacteria inoculant. The biocontrol bacterium BV03 is bacillus velezensis BV03 preserved in the China General Microbiological Culture Collection Center, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.18936, the preservation date is November 11, 2019, and the bacillus velezensis is classified and named as Bacillus velezensis. The bacteria inoculant has an obvious biological control effect on verticillium wilt of solanaceae plants and / or rosaceae plants, the control effect is close to 85% during seed disinfection, the control effect reaches 75% or above during root irrigation treatment, the control effect of reaches 70% or above during spraying to stems and leaves, a large amount of cost is saved, and the bacteria inoculant has great application value for cultivation of economic plants.

Description

technical field [0001] The invention relates to the technical field of microbial plant protection applications, in particular to a biocontrol bacterium BV03, its application, its agent and its preparation method. Background technique [0002] In recent years, green ecological agriculture has continued to develop and achieved considerable results. In addition, as people pay more and more attention to food health, the demand for vegetables and fruits has greatly increased. [0003] Verticillium wilt is a serious worldwide disease, because of its wide range of disease, strong prevalence, and high incidence, it is one of the most devastating diseases in the production process of fruits and vegetables. bacteria and Verticillium dahliae. Verticillium black and white and Verticillium dahliae not only spread through seeds, but also exist and survive in diseased soil and straw-returned soil for a long time. They can invade from plant roots and propagate upward with vascular bundles...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01N25/04A01P3/00C12R1/07
CPCC12N1/20A01N63/00A01N25/04C12R2001/07C12N1/205
Inventor 谢雪迎张新建周方园周红姿张广志赵晓燕吴晓青范素素王加宁
Owner ECOLOGY INST SHANDONG ACAD OF SCI
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