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Nano drug delivery system with mimic enzyme activity, drug-loaded nanoparticles and preparation method and application thereof

A nano drug delivery system, drug-loaded nano technology, applied in the direction of organic active ingredients, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2020-07-31
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there is no report about the nano-drug delivery system with mimic enzyme activity for the treatment of psoriasis

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  • Nano drug delivery system with mimic enzyme activity, drug-loaded nanoparticles and preparation method and application thereof
  • Nano drug delivery system with mimic enzyme activity, drug-loaded nanoparticles and preparation method and application thereof
  • Nano drug delivery system with mimic enzyme activity, drug-loaded nanoparticles and preparation method and application thereof

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preparation example Construction

[0034] The invention provides a preparation method of the nano drug delivery system, comprising the following steps:

[0035] 1) Ce(NO 3 ) 3 ·6H 2 Mix O, cyclodextrin, alkali and water, and react at 105-180°C for 4-12 hours to obtain a reaction liquid;

[0036] 2) Separating the reaction liquid from solid to liquid, and removing unreacted raw materials from the obtained solid phase to obtain a nano drug delivery system.

[0037] The present invention will Ce(NO 3 ) 3 ·6H 2 O, cyclodextrin, alkali and water are mixed and reacted at 105-180° C. for 4-12 hours to obtain a reaction liquid.

[0038] In the present invention, preferably Ce(NO 3 ) 3 ·6H 2O. Cyclodextrin is pre-dissolved in water, and then mixed with alkali. The mixed solution is preferably placed in a stainless steel hydrothermal reaction kettle for hydrothermal reaction. The temperature of the hydrothermal reaction is preferably 125-165° C., and the time of the hydrothermal reaction is preferably 6-10 h. ...

Embodiment 1

[0052] Preparation method of nano drug delivery system

[0053] Weigh Ce(NO 3 ) 3 ·6H 2 O 0.18685g, β-CD 2.270g and NaOH 0.2g were added to 30mL distilled water, ultrasonicated until dissolved, transferred to a stainless steel hydrothermal reaction kettle, reacted at 125°C for 6h, then cooled to room temperature, and used a high-speed centrifuge at 14000rpm Centrifuge for 15min, remove the supernatant, and wash three times with distilled water to obtain the nano drug delivery system (β-CD / CeO 2 NPs).

Embodiment 2

[0055] Preparation method of drug-loaded nanoparticles

[0056] Dissolve 0.5 mg of the drug dithranol in 100 μL of DMSO, and slowly add it dropwise to 1 mL of 1 mg / mL β-CD / CeO 2 In the nano-system of NPs, stir at room temperature for 24 hours in the dark, and centrifuge in a high-speed centrifuge at 14,000 rpm for 15 minutes to obtain a solid phase that is redispersed with distilled water and freeze-dried for later use. The drug content of the obtained supernatant is measured by high performance liquid chromatography. The encapsulation efficiency and drug loading were calculated according to the following formula a and formula b.

[0057] Encapsulation efficiency=(dosage amount-unloaded drug amount) / dosage amount×100% formula a

[0058] Drug loading = drug loading in nanoparticles / total mass of nanoparticles Formula b

[0059] It can be obtained by calculation that the drug-loading capacity of the drug-loaded nanoparticles is 28.1% and the encapsulation efficiency is 78%. ...

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Abstract

The invention provides a nano drug delivery system with mimic enzyme activity, drug-loaded nanoparticles and a preparation method and application thereof, and belongs to the technical field of drug delivery systems. The nano drug delivery system with the mimic enzyme activity comprises nano cerium dioxide and cyclodextrin, and the cyclodextrin is modified on the surface of the nano cerium dioxidethrough hydrogen bonds and complexing action force; and the nano drug delivery system is spherical, and the particle size is 50-75 nm. Based on the fact that the nano drug delivery system has variousmimic enzyme activities and has a function of entrapping drugs, the invention further provides the drug-loaded nanoparticles for treating psoriasis. The drug-loaded nanoparticles comprise the nano drug delivery system and the drugs; the drugs are loaded in a cyclodextrin cavity in the nano drug delivery system through the host-guest effect; and the particle size of the drug-loaded nanoparticles is50-200 nm. At present, it is considered that attack of psoriasis is closely related to generation of active oxygen, the mimic enzyme activity of the drug-loaded nanoparticles is achieved to remove active oxygen, and the drug-loaded nanoparticles and the loaded drugs jointly play a role in resisting psoriasis.

Description

technical field [0001] The invention belongs to the technical field of drug delivery systems, and in particular relates to a nanometer drug delivery system with simulated enzyme activity, drug-loaded nanoparticles and a preparation method and application thereof. Background technique [0002] In recent years, nano drug delivery systems have been widely used in various fields, and their application in the medical field has shown unique advantages. Researchers use different techniques and preparations to prepare different drug-loaded nanoparticles to exert corresponding therapeutic effects on diseases. The currently developed nano-drug delivery system has the advantages of sustained drug release, increased permeability and targeted drug delivery, but it does not have enzyme-mimicking activity and cannot effectively overcome the damage to tissue cells caused by active oxygen generated by the drug itself or the disease itself. [0003] As an immune-related inflammatory skin dis...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/52A61K31/122A61P17/06
CPCA61K31/122A61K47/52A61K47/6951A61P17/06
Inventor 丁德军张维芬李文通程妮刘国艳
Owner WEIFANG MEDICAL UNIV
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