External common-optical-path off-axis digital holographic microimaging device

Abstract

The invention relates to an external common-optical-path off-axis digital holographic microimaging device. The device sequentially comprises a one-dimensional periodic grating, a fourth lens, a filterand an imaging detector in the optical path direction. Parallel light beams carrying object information are diffracted after passing through the one-dimensional periodic grating, and on the Fourier spectrum surface of the fourth lens, zero-level diffracted light and + 1-level diffracted light filtered by a filter are adopted to generate an off-axis hologram on the target surface of the imaging detector, and the off-axis hologram is recorded by the imaging detector. The off-axis hologram is reconstructed by adopting a well-known numerical reconstruction algorithm in the field of digital holography to obtain the phase information of the measured object. The device is simple and compact in structure and convenient to install and adjust, can be directly used as external equipment of a commercial optical microscope to carry out quantitative microscopic imaging on micro-nano structure objects such as biological cells, and has a very strong market application prospect.

Description

technical field [0001] The invention belongs to the field of digital holographic microscopic imaging, in particular to an external common optical path off-axis digital holographic microscopic imaging device. Background technique [0002] Biological cells / tissues have three-dimensional distribution characteristics, and three-dimensional measurement is a necessary condition for understanding all information about tissue structure and activities. However, most of the cells / tissues in the culture process are unstained original cells / tissues, showing transparent or translucent properties. After the light passes through, only the phase changes, and the wavelength and light intensity (amplitude) are almost unchanged. When observing cells / tissues with a traditional optical microscope, only light intensity (two-dimensional) information can be seen, and phase (three-dimensional) information of cells / tissues cannot be obtained. Therefore, it is the goal pursued by the biomedical field...

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes an external common optical path off-axis digital holographic microscopic imaging device, which can be used for quantitative phase detection of tiny objects such as biological cells / tissues. The present invention solves the problem that existing optical microscopes can only obtain two-dimensional objects The limitation of light intensity information overcomes the technical problems of high coherent noise and difficult optical path adjustment in traditional coherent light illumination or partially coherent light illumination digital holographic imaging systems, and can realize high dynamic real-time measurement

Images