Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Genetic molecular marker related to pork quality electrical conductivity traits

A molecular marker and molecular genetic marker technology, applied in the field of molecular markers, can solve the problems of high computational intensity, overestimation of marker effects, unreasonable setting of significance thresholds, etc., and achieve high sensitivity, good pork quality, and high conductivity. Effect

Active Publication Date: 2020-07-28
广西扬翔股份有限公司 +1
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such methods often face problems such as high computational intensity, overestimation of marker effects, and unreasonable setting of significance thresholds.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Genetic molecular marker related to pork quality electrical conductivity traits
  • Genetic molecular marker related to pork quality electrical conductivity traits
  • Genetic molecular marker related to pork quality electrical conductivity traits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The basic research group in this example is 303 ternary cross pigs, all from Guangxi Yangxiang Agriculture and Animal Husbandry Co., Ltd., including 131 boars and 172 sows. The measurement site for electrical conductivity properties was the long dorsi muscle between the 13th and 14th intercostals, and the measurement was completed within 45 minutes after slaughter.

[0021] (1) Genotyping and quality control

[0022] Collect pig ear tissue samples or blood samples, extract total DNA, and use GGP 50k SNP (GeneSeek, US) chip for genotyping to obtain 50,679 SNP markers covering the entire genome. According to the latest version of the pig reference genome (Sscrofa11.1), the NCBI genome comparison program (https: / / www.ncbi.nlm.nih.gov / ) was used to update the physical location of all SNP markers. SNPs with unknown genomic location are not used for association analysis. For all SNP markers on autosomes, use Plink software for quality control. The standard is individual detectio...

Embodiment 2

[0037] According to the genetic results obtained from the above screening, molecular genetic markers related to the conductivity traits of pork are shown. This molecular genetic marker is located at the 59537927 nucleic acid site of pig chromosome 4, which is an A> The C mutation corresponds to the 51st nucleic acid site of SEQ ID NO. 1 in the nucleic acid sequence table.

Embodiment 4

[0039] Those skilled in the art can easily design primers for amplifying the molecular markers or probes for identifying the molecular markers according to the molecular genetic markers of the present invention, so as to be used for the detection of the genetic markers, for example by PCR amplification. The molecular genetic markers can be cloned and sequenced to obtain the corresponding sequences, or detected by Bsm-RFLP polymorphism. Therefore, the present invention also includes primers for amplifying the molecular genetic markers or probes for identifying the molecular genetic markers, and kits containing the primers or probes.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the technical field of molecular markers and particularly to a genetic molecular marker related to pork quality conductivity traits. The molecular marker can be used to breed pigs with higher pork conductivity. A breeding method is as follows: conducting sampling, extracting DNA and performing quality testing, performing genotyping to obtain SNP typing data, and obtaining the molecular marker related to the pork meat conductivity. A nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO:1, an A / C allelic mutation exists at a 51st base of the sequence, the nucleotide of the shown sequence produces polymorphism, and when the 51st nucleotide in the nucleotide sequence is C, pork has higher conductivity and better quality.

Description

【Technical Field】 [0001] The present invention relates to the technical field of molecular markers, in particular to a genetic molecular marker related to the conductivity traits of pork quality. 【Background technique】 [0002] my country is a big pig-raising country as well as a big pork consuming country. In the past few decades, people only paid attention to the growth traits of pigs, such as feed utilization, lean meat percentage, etc., but often ignored meat quality traits, leading to pork quality decline. With the improvement of people’s living standards, people’s requirements for pork quality have shifted from quantity to quality. Therefore, pork quality traits have become an important economic trait, which is more and more widely used by domestic and foreign pig raising companies and breeding pig improvement companies. attention. [0003] Electrical conductivity is one of the important meat quality traits that affect the freshness of pork. Nowadays, our country mainly uses...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124Y02P60/87
Inventor 赵云翔高广雄高宁李私丞朱琳郑伟
Owner 广西扬翔股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com