Lung cancer screening kit
A technology of kits and reagents, applied in the field of lung cancer screening kits, can solve problems such as the unrecognized relationship with ZNF808, and achieve low cost, less time-consuming, and effective detection
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[0090] Experimental example The relationship between ZNF808 methylation level and lung cancer
[0091] 1. Clinical data
[0092] 56 patients with lung cancer and 44 patients with benign lung diseases were selected. The basic information is as follows:
[0093]
[0094] 2. ZNF808 methylation sequencing
[0095] (1) Lung tissue DNA extraction: DNA was extracted from tissue samples using the tissue DNA extraction and purification kit QlAampDNA Mini Kit (article number: 51304) purchased from Qiagen;
[0096] (2) DNA concentration measurement and quality inspection: Take 1 μl to measure the concentration of Qubit, and print the label to paste the barcode; take 1 μl for 2100 quality inspection;
[0097] (3) Bisμlfite (bisulfite) conversion: the Bisμlfite conversion experiment was carried out using the methylation kit EZDNA Methylation-Lightning Kit (Cat. No.: D5030T) from Zymo research;
[0098] (4) Library construction: use the Ironman library construction kit from Guangzhou ...
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