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Screening and application of urine protein marker related to liver metastatic cancer

A liver metastasis and marker technology, applied in the field of clinical medicine, can solve the problems of poor sensitivity and specificity of tumor biomarkers and inability to predict liver cancer alone.

Inactive Publication Date: 2020-07-17
BEIJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, tumor biomarkers have poor sensitivity and specificity, and many studies have shown that biomarkers alone cannot predict liver cancer (see Hellstern S, Sasaki T, Fauser C, et al. Functional studies on recombinant domains of Mac-2 -bindingprotein[J].Journal of Biological Chemistry,2002,277(18):15690-15696.)

Method used

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  • Screening and application of urine protein marker related to liver metastatic cancer
  • Screening and application of urine protein marker related to liver metastatic cancer
  • Screening and application of urine protein marker related to liver metastatic cancer

Examples

Experimental program
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Embodiment 1

[0069] Example 1. Establishment of tumor animal models

[0070] 1. The walker256 rat tumor model is a classic animal model for studying tumor progression or tumor cachexia, and is suitable for studying pathophysiological and morphological changes during tumor progression.

[0071] Using this animal model to simulate the pathogenesis of tumors, observe the overall changes of tumors from normal, early tumors, tumor progression, and late tumor cachexia, which is helpful for early diagnosis of liver metastases, monitoring of liver metastases, and diagnosis of advanced tumor cachexia. important guiding significance.

[0072] 2. Materials and Reagents

[0073] 1) Instrument:

[0074] Rat metabolic cage: purchased from Beijing Jiayuan Xingye Technology Co., Ltd.

[0075] Thermo-Orbitrap Lumos is purchased from Thermo company;

[0076] 2) Main reagents:

[0077] Deionized water was from MillliQ RG ultrapure water system; chromatographic grade acetonitrile, formic acid and methano...

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Abstract

The invention relates to screening and application of a urine protein marker related to liver metastatic cancer. Specifically, the present invention relates to a method for establishing an animal model for screening urine protein markers related to liver metastatic cancer. The invention also relates to application of the urine protein marker related to liver metastatic cancer obtained by the method in preparation of a kit for diagnosing liver metastatic cancer in a subject at an early stage. The urine protein marker is selected from one or more of parvalbumin alpha, annexin A1, a polymer immunoglobulin receptor, lysosome thioesterase (PPT2), cobalamin protein-2, a cathepsin H precursor, a galectin-3 binding protein, an HLA-I tissue compatible antigen, beta-2 microglobulin, vascular cell adhesion protein-1, complement C4, alpha-1-acid glycoprotein, or a combination thereof.

Description

technical field [0001] The invention relates to clinical medicine; in particular, it relates to the screening of urine protein markers related to human liver metastasis and its application in diagnosis and treatment. Specifically, the present invention relates to the use of liver metastases in rat disease models and mass spectrometry proteomics technology to screen and obtain urine protein markers related to the diagnosis and / or monitoring of human liver metastases, and their use in diagnosis and treatment . Background technique [0002] Tumor markers can determine whether an individual is at risk of cancer, detect cancer early, predict survival and recurrence, monitor treatment effects, and more. Such tumor markers are mainly derived from body fluids or tissues and organs, and mainly include nucleic acids, proteins, metabolites, and the like. [0003] As one of the important sources of body fluids, urine plays an important role in the human body. Since urine lacks a mech...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/574A01K67/027
CPCA01K67/0271G01N33/574G01N33/57438G01N33/577G01N2800/08
Inventor 高友鹤张亚萌
Owner BEIJING NORMAL UNIVERSITY
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