Plant-bacterium-fungus-rhamnolipid combined remediation method for petroleum-contaminated soil

A technology of rhamnolipid and oil pollution, applied in the restoration of contaminated soil, etc., can solve the problems of destroying soil structure and components, high price, secondary pollution, etc., and achieve easy biodegradation, fast spreading, and strong vitality Effect

Inactive Publication Date: 2020-07-17
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heat treatment is through incineration or calcination, which can purify most of the organic pollutants in the soil, but at the same time, it also destroys the soil structure and components, and is expensive and difficult to implement
Chemical leaching and water washing can also achieve good degreasing effect, but the secondary pollution of the chemical reagents used limits its application

Method used

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  • Plant-bacterium-fungus-rhamnolipid combined remediation method for petroleum-contaminated soil
  • Plant-bacterium-fungus-rhamnolipid combined remediation method for petroleum-contaminated soil
  • Plant-bacterium-fungus-rhamnolipid combined remediation method for petroleum-contaminated soil

Examples

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preparation example Construction

[0037] (2) the preparation method of composite microbial bacterial agent comprises the steps:

[0038] 1) Cultivate Bacillus subtilis in LB medium until the number of colonies is greater than 1×10 10 cfu mL -1 ;

[0039] The composition of the LB medium is: 10 g of peptone, 5 g of yeast extract, 10 g of sodium chloride, 1 L of distilled water, pH=7.5, and sterilized at 121° C. for 20 min.

[0040] 2) Cultivate Pseudomonas fluorescens in beef extract peptone medium until the number of colonies is greater than 1×10 10 cfu mL -1 ;

[0041] The composition of the beef extract-peptone medium is: 5 g of beef extract, 10 g of peptone, 5 g of sodium chloride, 1 L of distilled water, pH=7.2-7.4, and sterilized at 121° C. for 20 minutes.

[0042] 3) Cultivate Streptococcus faecalis in MRS medium until the number of colonies is greater than 1×10 10 cfu mL -1 ;

[0043]The composition of the MRS medium is: casein 10g, beef extract 10g, yeast extract juice 5g, glucose 5g, sodium ac...

Embodiment 1

[0059] Taking the site of Xinkai village tire cracking oil refinery, Jiguanshan Town, Fengcheng City, Dandong as the test site, 7 test areas are set as table 1, and each test area is divided into 2 pieces, wherein one test area adopts the method of the present invention, and the other test area as a control experiment.

[0060] One, adopt the method of the present invention

[0061] A method for plant-bacteria-fungi-rhamnolipid joint restoration of oil-contaminated soil, comprising the steps of:

[0062] 1. Remove impurities on the surface of oil-contaminated soil;

[0063] 2, the preparation method of composite microbial bacterial agent comprises the steps:

[0064] 1) Put Bacillus subtilis in LB medium, at 30°C, 200r·min -1 Cultivate until the number of colonies is greater than 1×10 10 cfu mL -1 ;

[0065] The composition of the LB medium is: 10 g of peptone, 5 g of yeast extract, 10 g of sodium chloride, 1 L of distilled water, pH=7.5, and sterilized at 121° C. for 20...

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Abstract

The invention relates to a plant-bacterium-fungus-rhamnolipid combined remediation method for petroleum-contaminated soil. According to the technical scheme, impurities on the surface of petroleum-contaminated soil are removed, uniformly spreading the composite microbial agent on the petroleum-contaminated soil, uniformly mixing the composite microbial agent with the petroleum-contaminated soil with the depth of 10-20cm on the ground surface, uniformly spraying a rhamnolipid solution, sowing cynodon dactylon seeds, and covering with soil with thickness of 1-2 cm. By the adoption of the method,the petroleum-contaminated soil is remediated, and the natural ecological environment of local soil can be restored.

Description

technical field [0001] The invention relates to a method for jointly repairing oil-contaminated soil by using plants-bacteria-fungus-rhamnolipid, and belongs to the technical field of ecological method for repairing oil-contaminated soil. Background technique [0002] Before the 1980s, the treatment of petroleum hydrocarbon-contaminated soil was limited to physical and chemical methods, namely heat treatment and chemical leaching. Heat treatment is through incineration or calcination, which can purify most of the organic pollutants in the soil, but at the same time, it also destroys the soil structure and components, and is expensive and difficult to implement. Chemical leaching and water washing can also achieve good degreasing effect, but the secondary pollution of the chemical reagents used limits its application. Since the 1980s, the bioremediation technology of polluted soil has attracted more and more attention. Bioremediation is the process of using biological life ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10B09C1/08
CPCB09C1/08B09C1/10B09C1/105
Inventor 包红旭蒋大展王丽涛刘笑含高东宏徐晓红宋泽斌巨承文李泽刘洪源胡家伟金楠珣
Owner LIAONING UNIVERSITY
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