Preparation method and application of anti-tumor microparticles modified by genetic engineering

Abstract

The invention provides a preparation method of anti-tumor microparticles modified by genetic engineering. The preparation method comprises the following steps: S1, constructing recombinant plasmids for overexpression of GM-CSF and transmembrane expression of GM-CSF; S2, obtaining isolated tumor cells for primary tumor cell culture, and obtaining primary tumor cells; S3, packaging slow virus particles with overexpression of GM-CSF and transmembrane expression of GM-CSF; S4, infecting the primary tumor cells obtained in the step S2 to obtain primary tumor cells with overexpression of GM-CSF andtransmembrane expression of GM-CSF; S5, carrying out X-ray irradiation on the primary tumor cells obtained in the step S4 and a culture solution, and collecting the supernatant after radiotherapy to obtain a mixture of required microparticles and apoptotic tumor cell fragments; and S6, centrifuging the mixture obtained in the step S5. The invention can solve the technical problems of poor effect,large side effect, easy generation of drug resistance and inhibition of organism immunity of conventional local chemotherapy.

Description

technical field [0001] The invention relates to the field of anti-tumor technology, in particular to a preparation method and application of genetically engineered anti-tumor microparticles. Background technique [0002] Radiation therapy is an effective treatment method for routine clinical treatment of tumors, and tumor radiotherapy is a local treatment method that uses ionizing radiation generated by radiation to treat tumors. About 70% of tumor patients need radiotherapy during treatment, and about 40% of cancers can be cured with radiotherapy. The role and status of radiotherapy in tumor treatment is becoming more and more prominent, and it has become one of the main means of treating malignant tumors, especially in the treatment of some tumors such as head and neck cancer, breast cancer, lung cancer and cervical cancer. However, some tumors do not routinely receive radiotherapy in clinical practice, such as gastric cancer, colon cancer, bladder cancer, kidney cancer, ...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above problems, the present invention provides a preparation method, medicament and application of genetically engineered anti-tumor microparticles. The anti-tumor microparticles achieve anti-tumor effect through indirect radiotherapy and chemotaxis of immune cells into the tumor microenvironment , to solve the technical problems of poor effect of conventional local chemotherapy, large side effects, easy to produce drug resistance, and inhibition of the body's immunity

Images