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Set of early esophageal squamous cell carcinoma screening markers and application of markers in probe kit

A kit and cell cancer technology, applied in early esophageal squamous cell carcinoma screening markers and its application in primer probe kits, can solve endoscopic screening trauma, limited promotion, low efficiency, etc. problems, achieve high detection sensitivity and specificity, improve sensitivity and specificity, and achieve good technical reproducibility

Inactive Publication Date: 2020-07-10
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, endoscopic screening is invasive, costly, and inefficient, which limits the promotion of endoscopic screening in the early detection of esophageal squamous cell carcinoma in asymptomatic people

Method used

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  • Set of early esophageal squamous cell carcinoma screening markers and application of markers in probe kit
  • Set of early esophageal squamous cell carcinoma screening markers and application of markers in probe kit
  • Set of early esophageal squamous cell carcinoma screening markers and application of markers in probe kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Kit for screening early esophageal squamous cell carcinoma

[0035] 1. Composition of the kit

[0036] The composition of the kit is shown in Table 1, wherein the upstream primer combination consists of upstream primers corresponding to the five gene markers of SLAMF1, CRIM, CCSER1, MGARP, and POMK; the downstream primer combination consists of SLAMF1, CRIM, CCSER1, MGARP, The downstream primers corresponding to the five gene markers of POMK are composed respectively; the probe combination is composed of the probes corresponding to the five gene markers of SLAMF1, CRIM, CCSER1, MGARP and POMK respectively; the standard cDNA combination is composed of SLAMF1, CRIM, CCSER1, The standard cDNA compositions corresponding to the five gene markers MGARP and POMK respectively.

[0037]

[0038] The source or method of preparation of the reagent

[0039] 2.1 Design of upstream primer combination and downstream primer combination

[0040] The present invention use...

Embodiment 2

[0066] Example 2 Analysis of the effectiveness of the kit of the present invention in the detection of early esophageal squamous cell carcinoma

[0067] In this study, 428 subjects who were suspected of having early squamous cell carcinoma of the esophagus who underwent gastroscopy from Anyang Cancer Hospital from January 2019 to June 2019 were collected from small pieces of tissue under gastroscopy. Invented a kit for the detection and diagnosis of early esophageal squamous cell carcinoma. All 428 subjects suspected of having early esophageal squamous cell carcinoma underwent pathological biopsy to confirm the diagnosis. Among the pathological biopsy results, 246 patients were diagnosed as esophageal squamous cell carcinoma. The possibility of squamous cell carcinoma of the esophagus was clearly ruled out in the remaining 182 patients.

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Abstract

The present invention belongs to the technical field of bioengineering and biomedicine, and specifically relates to the field of oncology and more specifically to a set of early esophageal squamous cell carcinoma screening markers and an application of the markers in a primer probe kit. The present invention provides a primer composition and a probe composition for detecting SLAMF1, CRIM, CCSER1,MGARP, and POMK genes. Besides, the primer composition and probe composition are utilized to prepare the kit for detecting early esophageal squamous cell carcinoma. The detection sensitivity of the kit for the early esophageal squamous cell carcinoma reaches 82.11% and the specificity reaches 86.99%. The kit is suitable for large-scale screening of the early esophageal squamous cell carcinoma of asymptomatic people in a high-incidence area of the esophageal squamous cell carcinoma.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and biomedicine, specifically relates to the field of oncology, and more specifically relates to a group of screening markers for early esophageal squamous cell carcinoma and the application thereof in a primer probe kit. Background technique [0002] Esophageal squamous cell carcinoma is one of the most common malignant tumors in the world, and its mortality rate ranks sixth. More than half of the approximately 500,000 new cases of esophageal squamous cell carcinoma in the world occur in China every year, which is higher than that in Western countries 100 times. Significantly different from Western populations, the prominent epidemiological features of Chinese esophageal squamous cell carcinoma are significant regional distribution differences and obvious familial clustering. The Taihang Mountains at the junction of Henan, Hebei, and Shanxi provinces, Linzhou, Henan is the area with the h...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6851
CPCC12Q1/6851C12Q1/6886C12Q2600/158C12Q2521/107C12Q2531/113C12Q2563/107
Inventor 王立东李欣然赵学科宋昕雷玲玲周福有王伟李恩民李秀敏高社干秦艳茹郭海周
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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