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Method for preparing pha by fermentation with high-salt molasses as raw material

A molasses and high-salt technology, applied in the field of fermentation to prepare PHA, can solve problems such as difficult to meet the needs of environmental protection, achieve the effects of reducing additional operations and economic costs, improving fermentation efficiency, and reducing the addition of salt

Active Publication Date: 2020-09-01
COFCO NUTRITION & HEALTH RES INST +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the rapid development of the current society, green environmental protection has become the main theme of contemporary chemical products. It is becoming more and more difficult to meet the needs of environmental protection for the materials extracted from fossils in the past.

Method used

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  • Method for preparing pha by fermentation with high-salt molasses as raw material

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Experimental program
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Effect test

Embodiment approach

[0027] According to a preferred embodiment of the present invention, the high-salt molasses is high-salt cane molasses, based on dry weight, its salt content is more than 9% by weight, for example, 9-11% by weight, and the sugar content is 50% by weight Above, for example, 50-70% by weight, the sugar includes glucose, sucrose and fructose, wherein, in terms of total sugar, the content of glucose is 5-15% by weight, the content of sucrose is 75-85% by weight, and the content of fructose It is 5-12 weight percent.

[0028] According to the present invention, the high-salt molasses may be crude high-salt molasses, refined high-salt molasses, or syrup obtained after decolorization and dilution of refined high-salt molasses.

[0029] According to the present invention, the content of the high-salt molasses in the fermentation medium can be selected within a wide range, and it can completely or partially replace the carbon source (glucose) in the fermentation medium. According to a...

preparation example

[0090] This preparation example is used to illustrate the activation of fermentation strains

[0091] Seed medium: containing 8g / L yeast powder, 12g / L peptone and 55g / L sodium chloride.

[0092] Halomonas was inoculated in the seed medium for primary activation culture at 37°C and 200rpm, and cultivated to OD 600 Reach about 4, obtain first-grade seed liquid;

[0093] Inoculate the primary seed liquid into the seed medium with an inoculation amount of 10% by volume, carry out secondary activation culture at 37°C and 200rpm, and cultivate to OD 600 Reach about 4, obtain secondary seed liquid, obtain fermented seed liquid.

Embodiment 1

[0095] This embodiment is used to illustrate the method for preparing PHA by fermentation provided by the present invention

[0096] Fermentation medium: Relative to 1L of fermentation medium, the content of high-salt molasses 1 is 25mL, the content of yeast powder is 17g, the content of dipotassium hydrogen phosphate is 3.5g, the content of disodium hydrogen phosphate is 6.2g, and the content of magnesium sulfate The content is 0.25g, and the content of sodium chloride is 55g. Adjust the pH to 8.5.

[0097] Supplement 1: High-salt molasses 1 is used as the carbon source, yeast powder is used as the nitrogen source; the carbon-to-nitrogen ratio is 16:1. Among them, relative to 1L of the first nutrient substance, 1 640 mL of high-salt molasses, 108 g of yeast powder; the volume ratio of supplement 1 to fermentation medium was 9:100.

[0098] Supplement 2: High-salt molasses 1 is used as carbon source, yeast powder is used as nitrogen source; the ratio of carbon to nitrogen is...

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Abstract

The invention relates to the field of PHA fermentation, in particular to a method for preparing PHA by fermenting high-salt molasses as a raw material. The method comprises: under the condition that PHA can be fermented and produced, inoculating the PHA fermentation strain into a fermentation medium for fermentation; wherein, the fermentation medium contains high-salt molasses, based on the dry weight of high-salt molasses, the salt The content is 8-11% by weight, and the sugar content is 40-70% by weight. The method can effectively reduce the production cost of PHA, and the fermentation efficiency has also been improved.

Description

technical field [0001] The invention relates to the field of PHA fermentation, in particular to a method for preparing PHA by fermenting high-salt molasses as a raw material. Background technique [0002] With the rapid development of the current society, green environmental protection has become the main theme of contemporary chemical products, and it has become increasingly difficult for the fossil-derived materials in the past to meet the needs of environmental protection. It is under such a trend that the development and production of biodegradable materials is more urgent and necessary. PHA is a new type of biodegradable plastic raw material, which has similar material properties to traditional plastic products. PHA can be accumulated and stored as an energy-supplying substance in most microorganisms, and can be produced, extracted and purified on a large scale by means of biological fermentation. [0003] In most PHA fermentation processes, single sugars—mainly monos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/62C12R1/01
CPCC12N1/20C12P7/625
Inventor 佟毅李义唐堂李大勇刘海军陈博彭超周卫强吴琼陈国强叶健文武丽达安泰
Owner COFCO NUTRITION & HEALTH RES INST
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