Method for improving Abelia seedling salt tolerance by oriented induction
A technology of directional induction and hexagram, which is applied in the field of obtaining hexagram seedlings to improve salt and alkali resistance, can solve the problems of high salt content, low survival rate of hexagram seedlings colonization, etc., and achieve the effect of high salt tolerance
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Embodiment 1
[0045] Embodiment 1 Improving Rodwood "Edward" "(Abelia 'Edward Goucher') is the method for salt tolerance
[0046]1. Disinfection of materials: Take the young branches of Liudaomu "Edward", cut them into about 4-5cm stems with axillary buds, and first wash them in washing liquid with washing powder (washing liquid refers to the mass fraction containing about 0.5% dodecyl Sodium benzenesulfonate aqueous solution) for 5-6 minutes, then rinse with tap water for 2-3 hours, then cut off the unfolded leaves, and cut into about 1.5cm bud stem segments. On the ultra-clean workbench, soak in 75% alcohol solution for 20 seconds, then transfer to 0.1% mercuric chloride solution for sterilization for 8-10 minutes, and rinse with sterile water 5-8 times.
[0047] 2. Induction of adventitious buds: the sterilized stems were cultured on the medium of MS + kinetin 1.0 mg / L + indole butyric acid 0.1 mg / L + 3% sucrose + 0.1% NaCl 40-50 days. During the bud induction process, the stem segment...
Embodiment 2
[0057] Example 2 The method for improving the salt tolerance of Abelia grandiflora (scientific name: Abelia grandiflora "Francis Mason")
[0058] 1. Disinfection of materials: Take the young branches of Hexaphyllum mosaicus, cut them into about 4-5cm stems with axillary buds, and first wash them in washing liquid with washing powder (washing liquid refers to dodecylbenzene with a mass fraction of 0.5%) sodium sulfonate aqueous solution) for 5-6 minutes, then rinse with tap water for 3 hours, then cut off the unfolded leaves and cut them into 1.5cm budded stem segments. On the ultra-clean workbench, soak in 75% alcohol solution for 20 seconds, then transfer to 0.1% mercury solution for sterilization for 8-10 minutes, and rinse with sterile water 6-8 times.
[0059] 2. Induction of adventitious buds: the sterilized stems were cultured on the medium of MS + kinetin 1.0 mg / L + indole butyric acid 0.1 mg / L + 3% sucrose + 0.1% NaCl 40-50 days. During the bud induction process, the...
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