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Brevibacillus laterosporus and composition thereof and application of Brevibacillus laterosporus and composition

A technology of Bacillus brevius and antibacterial composition, applied in the field of biological feed additives, can solve problems such as environmental pollution, destruction of intestinal microorganisms, and drug-resistant bacteria, and achieve the effects of environmental friendliness, broad application prospects, and safety for humans and animals

Active Publication Date: 2020-07-10
中粮生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The harm of misuse of antibiotics is extremely serious. It will not only lead to the emergence of drug-resistant bacteria, but also cause various problems such as the decline in the use of antibiotics in the human body, the destruction of intestinal microorganisms, the inhibition of the hematopoietic ability of animal larvae, and environmental pollution.

Method used

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  • Brevibacillus laterosporus and composition thereof and application of Brevibacillus laterosporus and composition
  • Brevibacillus laterosporus and composition thereof and application of Brevibacillus laterosporus and composition
  • Brevibacillus laterosporus and composition thereof and application of Brevibacillus laterosporus and composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Isolation and screening of the departure bacterial strain of Brevibacillus spp.

[0044]According to the document "Isolation and structural elucidation of brevibacillin, anantimicrobial lipopeptide from Brevibacillus laterosporus that combats drug-resistant gram-positive bacteria", Applied and environmental microbiology (2016), a strain that was isolated from the soil of Fuhai County, Xinjiang, and screened for the Staphylococcus aureus ATCC26001 has a better inhibitory effect on the wild-type Brevibacillus sporogenes (original strain).

[0045] The obtained bacterial strain was identified by 16S rDNA and physiological and biochemical characteristics (with reference to "Berger's Bacteria Identification Handbook", Science Press, 1984, the standard recorded in 1984 was identified), it was determined that it was Brevibacillus lateralsporosa, and it was used as a starting point. strains for subsequent experiments.

Embodiment 2

[0046] Example 2: Mutation of wild-type Brevibacillus spp. and mutant strain screening

[0047] Utilize atmospheric pressure room temperature plasma (ARTP) mutagenesis technique to carry out mutagenesis to the wild-type Brevibacillus lateralsporosa obtained in embodiment 1, then through high-throughput screening to obtain Staphylococcus aureus ATCC26001 that has obvious inhibitory effect Brevibacillus sporogenes B8. Specific steps are as follows:

[0048] 1) Pick out a single colony of the originating strain and inoculate it into fresh LB liquid medium, culture at 37°C for 24 hours, transfer to fresh LB liquid medium, and cultivate to the OD of the bacterial liquid 600 for 2-3;

[0049] 2) Dilute the above bacterial solution to OD with sterile water 600 1, absorb 10 μL of the diluted bacterial solution and evenly spread it on the slide, use the atmospheric pressure room temperature plasma (ARTP) mutagenesis breeding instrument for mutagenesis, place the above slide under th...

Embodiment 3

[0054] Embodiment 3: the cultivation and antibacterial activity assay of Brevibacillus lateralsporosa B8

[0055] 3-1 Spray-dried powder and antibacterial activity of the culture of Brevibacillus sporogenes B8

[0056] Adopt the following steps to prepare the culture of the Brevibacillus sporogenes B8 that embodiment 2 obtains into spray-dried powder:

[0057] 1) Transfer the strain of Brevibacillus sporogenes B8 into fresh LB medium at an inoculum size of 0.2v / v%, cultivate overnight at 37°C, and then insert the obtained culture solution into the above-mentioned medium at 1v / v%. In the medium of Brevibacillus sporogenes, culture at 200rpm at 37°C for three days to obtain the fermentation broth; take out 1mL of the fermentation broth and centrifuge at room temperature (8000rpm, 10min) to obtain the supernatant of the fermentation broth

[0058] 2) Boil the remaining fermentation broth at 100°C for 15 minutes, spray dry to obtain spray-dried powder (conditions for spray drying...

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Abstract

The invention discloses a strain of brevibacillus laterosporus B8 and a composition comprising the same and an application of the brevibacillus laterosporus B8 and the composition. The brevibacillus laterosporus B8 disclosed by the invention has a potent inhibitory effect on animal pathogens, such as staphylococcus aureus, can secrete antibacterial peptides, can be made into an antibacterial agentand has a good prevention and treatment effect on gram-negative bacteria and gram-positive bacteria, so as to replace antibiotics to play an antibacterial role in a feed and reduce use of the antibiotics in the biological feed field; and furthermore, the brevibacillus laterosporus B8 disclosed by the invention does not have toxicity or pathogenicity, is safe for people and animals and friendly toenvironment, is a useful resource for research and development of a biological feed and has a potential application prospect.

Description

technical field [0001] The invention relates to the field of biological feed additives, in particular, the invention relates to a Brevibacillus laterosporus (Brevibacillus laterosporus) with antibacterial effect, an antibacterial composition comprising the same and uses thereof. Background technique [0002] As one of the greatest inventions in the medical field in the 20th century, antibiotics have played an extremely important role in the prevention and treatment of various diseases. As a major producer of antibiotics, China uses antibiotics particularly frequently. It is estimated that the annual production of antibiotic raw materials in my country is about 210,000 tons, of which about 97,000 tons are used in animal husbandry. Since it was discovered in the 1950s that the use of low-concentration antibiotics in feed can not only prevent diseases, but also promote the growth of livestock and poultry, various antibiotics have been widely added to feed, and the abuse of ant...

Claims

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Application Information

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IPC IPC(8): C12N15/20A01N63/20A01P1/00C12R1/13
CPCC12R2001/13C12N1/205
Inventor 杨鑫朱峰华安泰陈博王靖臧传刚朱威宇焦琳郑晓卫金渭武
Owner 中粮生物科技(北京)有限公司
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