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A kind of cellulomonas and method and application thereof for preparing microbial flocculant

A technology of microbial flocculant and cellulomonas, which is applied in the biological field, can solve the problems of high production cost and unstable flocculation performance, and achieve the effects of cost saving, good flocculation performance and low dosage

Active Publication Date: 2021-11-19
盘锦检验检测中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the industrial production process of microbial flocculants in my country, there are generally problems such as unstable flocculation performance and high production costs. Therefore, the lack of high-yield and excellent performance flocculant strains is still limiting the industrialization process of microbial flocculants in my country. main element

Method used

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  • A kind of cellulomonas and method and application thereof for preparing microbial flocculant
  • A kind of cellulomonas and method and application thereof for preparing microbial flocculant
  • A kind of cellulomonas and method and application thereof for preparing microbial flocculant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Isolation and Identification of Strain 40-2

[0043] 1. Isolation and purification of strain 40-2

[0044] The samples used in this embodiment are collected from animal hair, feces, and blood. The screening of Cellulomonas 40-2 adopts the plate streaking method, wherein the liquid culture conditions are 37 ° C, the shaking table speed is 155 rpm, and the solid plate culture conditions are Culture in a biochemical incubator at 37°C. Concrete separation and purification are carried out according to the following steps:

[0045] 1) Enrichment, isolation and purification of strains: under sterile conditions, take 1.0 g of the sample and put it into a 10 mL plastic centrifuge tube containing 5 mL of enrichment medium, and incubate at 37°C for 24 hours. The culture solution was streaked on the surface of LB solid medium, and cultured at 37°C for 48h. Pick a yellow single colony with a smooth and viscous surface, streak and purify it multiple times, and store it in a magnet...

Embodiment 2

[0062] Preparation process of microbial flocculant produced by Cellulomonas 40-2 strain

[0063] Cellulomonas 40-2 was inoculated into the fermentation medium, the culture temperature was 37°C, and the shaker was cultivated at 155rpm for 48h to obtain the fermentation broth containing the microbial flocculant, centrifuged at 8000rpm for 5min, the supernatant was discarded, and the bacterial precipitate was retained , the precipitate was dissolved and resuspended in PBS, washed twice, centrifuged at 8000r / min for 5min, and then the precipitated bacteria were resuspended in PBS solution, and then ultrasonically crushed by a cell ultrasonic breaker. The broken liquid obtained after half an hour was the microbial flocculant.

[0064] Specifically, in this embodiment, the composition of the fermentation medium involved is (per liter): CMC-Na 10g, KH 2 PO 4 1g, K 2 HPO 4 1g, MgSO 4 0.2g, (NH 4 ) 2 SO4 3g, NaCl1g distilled water 1000mL, pH7.0-8.0, sterilized at 121°C for 30m...

Embodiment 3

[0066] Study on the distribution of flocculation activity produced by Cellulomonas 40-2 strain

[0067] Prepare kaolin suspension (4g / L) as simulated wastewater to test the flocculation performance of microbial flocculants. The specific method is: put 100mL kaolin suspension (4g / L) in a 150mL beaker, add 1mL microbial flocculant, stir rapidly at room temperature for 1min, stir slowly for 2min, and stand still for 10min. The absorbance was measured at a wavelength of 550nm by a spectrophotometer, and distilled water was used to replace the microbial flocculant as a control group.

[0068] The formula for calculating the flocculation rate is: flocculation rate=(A-B) / A×100% where A is the absorbance of the control group at 550nm, and B is the absorbance of the experimental group at 550nm. Calcium chloride is a common coagulant aid for microbial flocculants, use 10% CaCl 2 1mL was used as a coagulant aid, and the kaolin suspension was added together with the microbial flocculant...

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Abstract

The invention discloses a cellulomonas and a method and application for preparing a microbial flocculant by using the cellulomonas. The invention solves the problems of high production cost of the existing microbial flocculant and secondary pollution after flocculation, and the newly discovered strain The classification of 40‑2 is named as Cellulomonas livestock sp. 40‑2, Cellulomonas livestock 40‑2 has been preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, and the preservation number is CGMCC No.1.17732 . The advantages of the present invention are as follows: 1. Discover a new species of Cellulomonas - Cellulomonas 2. The microbial flocculant prepared by Cellulomonas has good flocculation performance; 3. The microbial flocculant produced is efficient, safe and It is non-toxic and does not produce secondary pollution; 4. The fermentation time of the microbial flocculant produced is short, the dosage is low, and the cost is saved. In addition, the present invention relates to that the microbial flocculant has great application potential and good application prospect in treating wastewater from livestock farms.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cellulomonas and a method and application thereof for preparing a microbial flocculant. Background technique [0002] Although traditional flocculants have many advantages in terms of flocculation performance and economic cost, they are easy to cause secondary pollution and have great hidden dangers in terms of safety. For example: Although traditional flocculants have many advantages in terms of flocculation performance and economic cost, aluminum salt flocculants are widely used in agriculture and forestry, causing aluminum damage to plants and seriously threatening the normal growth of plants. At the same time, these crops enter the human body and can affect human health. Diseases that often appear in medicine include aluminum encephalopathy, bone disease, and anemia, among which the dementia symptoms of the elderly are a kind of encephalopathy; iron salt flocculant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/06C02F3/34C12R1/01C02F103/20
CPCC02F3/34C02F2103/20C12N1/066C12N1/20C12N1/205C12R2001/01
Inventor 徐健峰章沙沙孙晓丽柳增善卢士英郭微
Owner 盘锦检验检测中心
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