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Method for enriching and separating efficient phosphorus-accumulating bacteria in active sludge of biological membrane reactor

A biofilm reactor and activated sludge technology, applied in the direction of using microorganisms, separating microorganisms, etc., can solve the problems of heavy workload, long time consumption, and low proportion of phosphorus-accumulating bacteria, so as to reduce the interference of nutrient competition and improve Species richness, effects of increased exposure

Active Publication Date: 2020-07-10
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method takes a long time, has a large workload, and the ratio of the screened phosphorus accumulating bacteria to the total isolated bacteria is low, and the pertinence is poor.
In addition, the acclimatization and enrichment conditions of the reactor are quite different from the nutrient status of the separation medium, and the growth of some phosphorus accumulating bacteria is affected by the metabolites of other bacteria and are eliminated, resulting in a decrease in the diversity of isolated phosphorus accumulating bacteria

Method used

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  • Method for enriching and separating efficient phosphorus-accumulating bacteria in active sludge of biological membrane reactor
  • Method for enriching and separating efficient phosphorus-accumulating bacteria in active sludge of biological membrane reactor
  • Method for enriching and separating efficient phosphorus-accumulating bacteria in active sludge of biological membrane reactor

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Effect test

Embodiment 1

[0047] 1. Pretreatment of sludge:

[0048] Take 50mL of activated sludge from a biofilm reactor in good operating condition, vortex for 2min, centrifuge at 12000rpm for 30s, discard the supernatant, repeat twice, and then vortex for 2min for acclimatization and cultivation to ensure the separation of bacteria and sludge. full contact with

[0049] 2. In vitro acclimatization and enrichment of strains:

[0050]Pour the above sludge into a 250mL Erlenmeyer flask filled with 100mL anaerobic liquid medium, shake and mix well, fill it with nitrogen gas to reach anaerobic state, and let it stand at room temperature for 24h. Centrifuge the mixed liquid in the cone (6000rpm, 20min), discard the supernatant, pour the sludge sediment into the Erlenmeyer flask filled with aerobic liquid medium, seal it with a breathable filter membrane, shake it at room temperature for 23h (30±0.5 ℃, 150rpm), let stand for 1h. Repeat the above cycle of domestication and enrichment, while monitoring th...

Embodiment 2

[0081] 1. Pretreatment of sludge:

[0082] Take 50mL of biofilm reactor activated sludge in good operating condition;

[0083] 2. In vitro acclimatization and enrichment of strains:

[0084] Pour the above sludge into a 250mL Erlenmeyer flask filled with 100mL anaerobic liquid medium, shake and mix well, fill it with nitrogen gas to reach anaerobic state, and let it stand at room temperature for 24h. Centrifuge the mixed liquid in the cone (6000rpm, 20min), discard the supernatant, pour the sludge sediment into the Erlenmeyer flask filled with aerobic liquid medium, seal it with a breathable filter membrane, shake it at room temperature for 23h (30±0.5 ℃, 150rpm), let stand for 1h. Repeat the above acclimatization and enrichment cycle while monitoring the phosphorus concentration in the supernatant. When the ratio of aerobic phosphorus uptake / anaerobic phosphorus release decreases, the cycle is stopped and separation is performed; Cycle domestication and enrichment, the rat...

Embodiment 3

[0112] 1. Pretreatment of sludge:

[0113] Take 50mL of activated sludge from a biofilm reactor in good operating condition, vortex for 3min, centrifuge at 12000rpm for 30s, discard the supernatant, repeat 3 times, and then vortex for 3min for acclimation and cultivation to ensure the separation of bacteria and sludge. full contact with

[0114] 2. In vitro acclimatization and enrichment of strains:

[0115] Pour the above sludge into a 250mL Erlenmeyer flask filled with 100mL anaerobic liquid medium, shake and mix well, fill it with nitrogen gas to reach anaerobic state, and let it stand at room temperature for 24h. Centrifuge the mixed liquid in the cone (6000rpm, 20min), discard the supernatant, pour the sludge sediment into the Erlenmeyer flask filled with aerobic liquid medium, seal it with a breathable filter membrane, shake it at room temperature for 23h (30±0.5 ℃, 150rpm), let stand for 1h. Repeat the above acclimatization and enrichment cycle while monitoring the p...

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Abstract

The present invention provides a method for enriching and separating efficient phosphorus-accumulating bacteria in active sludge of a biological membrane reactor, and relates to the technical field ofphosphorus-accumulating bacteria domestication and enrichment. The method comprises pretreatment of the sludge, in vitro domestication and enrichment of the bacteria and separation of the bacteria. By the method, the interference of mixed bacteria on phosphorus-accumulating bacteria nutrition competition can be reduced, and the species richness of the separated phosphorus-accumulating bacteria isimproved; a dilution solution with the OD600 value of 0.1-0.5 is used for four-region streak inoculation, so that the workload can be significantly reduced; and four-region streaking and chromogenicscreening are carried out synchronously, and by two rounds of chromogenic screening, screening of the phosphorus-accumulating bacteria is more accurate, the screening range is narrowed, the workload is reduced and the accuracy of screening is improved.

Description

technical field [0001] The invention belongs to the technical field of domestication and enrichment of phosphorus-accumulating bacteria, and in particular relates to a method for enriching and separating high-efficiency phosphorus-accumulating bacteria from biofilm reactor activated sludge. Background technique [0002] Biological phosphorus removal is an important phosphorus removal technology in sewage treatment. The biofilm method occupies an important position in the biological phosphorus removal technology because of its advantages of less sludge output, no need for sludge discharge, and small footprint. The biofilm method reduces the phosphorus concentration in the water through the aerobic phosphorus accumulation bacteria in the sludge, and anaerobic phosphorus release enriches the phosphate solution to achieve the simultaneous removal and enrichment of phosphate. It can be seen that the type and quantity of microorganisms in the biofilm directly affect the removal an...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12N1/36
CPCC12N1/02C12N1/36
Inventor 倪敏潘杨陈越张星宇曾斌斌
Owner SUZHOU UNIV OF SCI & TECH
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