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Nucleic acid composition for detecting virulence genes of helicobacter pylori, reagent and reagent kit including nucleic acid composition, and application of nucleic acid composition

Technology of a nucleic acid composition, Helicobacter pylori, applied in the field of biology

Active Publication Date: 2020-07-07
重庆博利达医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are many virulence genes of Helicobacter pylori detected, including vac A, vag A, Oip A, and Dup A. At present, the most commonly used detection method is the real-time PCR method. Although there are methods for simultaneous detection of multiple virulence genes, However, this method relies on both real-time fluorescent quantitative PCR instrument and mass spectrometer

Method used

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  • Nucleic acid composition for detecting virulence genes of helicobacter pylori, reagent and reagent kit including nucleic acid composition, and application of nucleic acid composition
  • Nucleic acid composition for detecting virulence genes of helicobacter pylori, reagent and reagent kit including nucleic acid composition, and application of nucleic acid composition
  • Nucleic acid composition for detecting virulence genes of helicobacter pylori, reagent and reagent kit including nucleic acid composition, and application of nucleic acid composition

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Embodiment 1

[0070] This embodiment provides a kit for detecting the virulence gene of Helicobacter pylori, which includes: positive control substance, negative control substance, PCR buffer, MgCl 2 solutions, dNTPs, hot-start DNA polymerases, UNG enzymes, and nucleic acid compositions.

[0071] Among them, the nucleic acid composition includes nucleic acid combinations 1-4, synthesized by Sangon Bioengineering (Shanghai) Co., Ltd., and its specific sequence is shown in Table 1.

[0072] Table 1 Sequence information of nucleic acid combinations 1-4

[0073]

[0074]

[0075] Positive controls were plasmid mixtures including all target gene targets (4 virulence genes: vacA s1 type, vacA m1 type, cagA and babA2).

[0076] The negative control substance was nuclease-free ultrapure water.

Embodiment 2

[0078] This embodiment provides the kit provided in embodiment 1 to detect or analyze the virulence gene of Helicobacter pylori, which specifically includes the following steps.

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Abstract

The invention discloses a nucleic acid composition for detecting virulence genes of helicobacter pylori, a reagent and reagent kit including the nucleic acid composition, and an application of the nucleic acid composition, and relates to the technical field of biology. Specifically, the nucleic acid composition comprises one or more combinations of nucleic acid compositions 1-4, and the nucleic acid compositions 1-4 are respectively used for detecting virulence genes vacA s1 type, vacA m1 type, cagA and babA2 of the helicobacter pylori. The nucleic acid composition can detect the virulence genes of the helicobacter pylori quickly and efficiently, and has the advantages of being high in detection sensitivity and good in detection specificity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nucleic acid composition for detecting the virulence gene of Helicobacter pylori and its reagent, kit and application. Background technique [0002] Helicobacter pylori (H.Pylori), a microaerophilic Gram-negative helicobacter, was listed by the World Health Organization as a class I carcinogen related to gastric cancer in 1994. Studies have shown that Helicobacter pylori infection is closely related to the occurrence and development of gastric inflammation, gastric mucosal atrophy, intestinal metaplasia, dysplasia, and gastric cancer. [0003] More than half of the world's population is infected with H.pylori, and about 20% of those infected suffer from secondary digestive diseases. Studies have shown that the pathogenesis of H.Pylori is related to the load of the bacteria itself, the gene polymorphisms of various virulence factors produced, and the living environment and lifestyl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/01
CPCC12Q1/689Y02A50/30
Inventor 唐彬何晓奕邓玲冯宇阳刘文正卜姣解庆华张雨
Owner 重庆博利达医学科技有限公司
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