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Mixed gel with anticoagulation effect, and preparation method and application thereof

A hybrid gel and anti-coagulation technology, applied in the field of 3D printing, can solve the problems of fixed molding method, main material without anti-coagulation effect, and less molding material.

Inactive Publication Date: 2020-07-03
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 3D printing, as a popular molding method in current life, production and scientific research, has a variety of molding methods, and the defects brought by these molding methods are the problems of less molding materials and fixed molding methods; and for the field of biomedical engineering, The main material applied to the 3D printing method is generally mixed with some proteins or co-printed with cells, and the main material does not have anticoagulant effect

Method used

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  • Mixed gel with anticoagulation effect, and preparation method and application thereof
  • Mixed gel with anticoagulation effect, and preparation method and application thereof
  • Mixed gel with anticoagulation effect, and preparation method and application thereof

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preparation example Construction

[0026] The embodiment of the present invention provides a method for preparing a mixed gel with anticoagulant effect, comprising the following steps:

[0027] Preparation of S1, methacrylic anhydride modified gelatin (GelMA)

[0028] Gelatin contains a cell adhesion peptide sequence-RGD that can promote cell adhesion, has good biocompatibility, and can be well applied in the field of biomedical engineering. Specifically, please refer to figure 1 The methacrylic anhydride-modified gelatin is obtained by reacting pigskin gelatin and methacrylic anhydride, the raw material is easy to obtain, and the prepared gelatin material has excellent biocompatibility.

[0029] In other embodiments, commercially available methacrylic anhydride-modified gelatin can also be used.

[0030] Preferably, the preparation method of methacrylic anhydride-modified gelatin comprises: mixing pigskin gelatin and phosphate buffered saline at a temperature of 50-70°C to obtain a reaction solution, adding ...

Embodiment 1

[0053] This embodiment provides a method for preparing a mixed gel with anticoagulant effect, comprising the following steps:

[0054] (1) Synthesis of methacrylamide-based gelatin (GelMA)

[0055] Weigh 5 g of pigskin gelatin and mix it into 50 mL of PBS (phosphate buffered saline 10% w / v) and stir at 60° C. until completely dissolved. Add 1 mL of MA (methacrylic anhydride, C 8 h 10 o 3 , 154.16Da) was added into the current solution at a rate of 0.5mL / min and stirred at 50°C for 3h. Then add 4 times the volume of PBS solution at 50°C and keep stirring to terminate the reaction. The diluted solution is placed in a 14kDa dialysis bag for dialysis (7d), during which the solution is constantly changed and stirred, and the dialysis bag is taken out after seven days. The solution was transferred to a centrifuge tube, centrifuged at 2500 rpm for 5 min, and the supernatant was taken out and freeze-dried to obtain white foamy modified gelatin.

[0056] (2) Preparation of GelMA-A-...

Embodiment 2

[0061] This embodiment provides a method for preparing a mixed gel with anticoagulant effect, comprising the following steps:

[0062] (1) Synthesis of methacrylamide-based gelatin (GelMA)

[0063] Weigh 5g of pigskin gelatin and mix it into 50mL of PBS (phosphate buffered saline 10% w / v) and stir at 50°C until completely dissolved. Add 1mL of MA (methacrylic anhydride, C 8 h 10 o 3 , 154.16Da) was added into the current solution at a rate of 0.4mL / min and stirred at 50°C for 4h. Then add 4 times the volume of PBS solution at 50°C and keep stirring to terminate the reaction. The diluted solution is placed in a 14kDa dialysis bag for dialysis (6d). The solution was transferred to a centrifuge tube, centrifuged at 2000 rpm for 8 min, and the supernatant was taken out and freeze-dried to obtain white foamy modified gelatin.

[0064] (2) Preparation of GelMA-A-H gel precursor liquid

[0065] Add 0.01 g of oxidized sodium alginate (A) and 0.01 g of heparin (H) to 1 mL of PBS s...

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Abstract

The invention discloses a mixed gel with an anticoagulation effect, and a preparation method and an application thereof, and relates to the technical field of 3D printing. The mixed gel with the anticoagulation effect comprises a photo-curing polymer formed by methacrylic anhydride modified gelatin, functionalized anticoagulant molecules are covalently connected to the light-cured polymer, the functionalized anticoagulant molecules are a product obtained after amidation chemical reaction and covalent crosslinking of heparin and alginate, the mixed gel with a slow release effect and the anticoagulant effect is formed, and the mixed gel can be applied to light-cured 3D printing. The preparation method of the mixed gel with the anticoagulation effect comprises the following steps: the methacrylic anhydride modified gelatin, alginate and heparin are subjected to a photocuring reaction and then subjected to a covalent cross-linking reaction. The finally formed photocuring polymer contains heparin and alginate, so the anticoagulation effect is achieved, and heparin can be slowly released in the application process to solve the burst release problem to a large extent.

Description

technical field [0001] The invention relates to the technical field of 3D printing, and in particular to a mixed gel with anticoagulant effect, its preparation method and application. Background technique [0002] 3D printing technology (Three-dimensional printing) is a new type of rapid prototyping technology. This technology has been used in daily processing, aerospace, biomedical engineering and other fields. At present, there are many 3D molding methods, but the basic principle All are inseparable from "accumulation layer by layer, layer by layer accumulation manufacturing". [0003] 3D printing, as a popular molding method in current life, production and scientific research, has a variety of molding methods, and the defects brought by these molding methods are the problems of less molding materials and fixed molding methods; and for the field of biomedical engineering, The main material applied to the 3D printing method is generally mixed with some proteins or co-print...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08J3/24C08J3/28C08L89/00C08L5/04C08L5/10C08H1/00B33Y70/00
CPCB33Y70/00C08H1/00C08J3/075C08J3/246C08J3/28C08J2389/00C08J2405/04C08J2405/10
Inventor 赵安莎高缘丁艺蕾杨苹
Owner SOUTHWEST JIAOTONG UNIV
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