Slide preparation method of bacterial staining method and application of slide preparation method

Abstract

The invention provides a slide preparation method of a bacterial staining method and application of the slide preparation method, the slide preparation method comprises: taking a clean glass slide, respectively dropwise adding a drop of sterile normal saline at two adjacent positions, and picking a small amount of plant bacteria into one drop of normal saline by using an inoculating loop for sterile operation; scribing and draining to another drop of normal saline by using an inoculating loop, so that the bacterial cell freely enter a second drop of normal saline from a first drop of normal saline, and the bacterial cell are diluted and then freely and uniformly dispersed; finally, scribing and cutting off water flow between the two drops of normal saline, so that water of the two drops ofnormal saline is prevented from flowing back and forth. According to the method, bacterial cells are dispersed more uniformly, the bacterial cell overlapping phenomenon caused by too thick smear fixation can be prevented, meanwhile, the drying and fixing time is short, the operation is convenient, the false positive and false negative phenomena are reduced, and the accuracy of staining observation can be greatly improved.

Description

Technical field [0001] The invention relates to the field of microbial staining, in particular to a method for preparing a bacterial staining method and its application. Background technique [0002] Bacteria are prokaryotic organisms. To observe their size and morphology clearly, they need to be stained and magnified by a microscope because of their small cells. Commonly used bacterial staining methods include single staining, counterstaining and differential staining. The differential staining methods mainly include Gram staining, methylene blue staining, acid-fast staining, Wright staining and Giemsa staining. The smearing, staining, and film production of bacteria are mainly divided into traditional manual operation and automatic operation with equipment. Due to the traditional manual operation of smearing, dyeing, and film production, it requires less equipment and less investment. Therefore, the application is more extensive and common, such as school teaching. [0003] T...

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Problems solved by technology

[0004] In the conventional Gram staining method, for beginners, if the smear is unevenly dispersed in the preparation step, the smear will be too thick, which will easily cause false positives and affect the observation of the bacterial morphology; natural air drying is used in the drying step It is a waste of waiting time and prolongs the entire staining operation time; if the alcohol lamp is used for drying and fixing with slight heat, it is easy for beginners to break the glass slide or cause false negatives due to excessive heating and fixing, and heating and fixing cannot be used for flagella staining and parasporal crystal staining

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