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Micro-fluidic chip applied to capture and screening of single cells, and application thereof

A microfluidic chip and single-cell technology, applied in specific-purpose bioreactors/fermenters, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as undiscovered cell capture technology reports

Inactive Publication Date: 2020-06-30
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, there is no related technical report that can integrate cell capture, multiple identification, culture and release on the same chip

Method used

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  • Micro-fluidic chip applied to capture and screening of single cells, and application thereof
  • Micro-fluidic chip applied to capture and screening of single cells, and application thereof
  • Micro-fluidic chip applied to capture and screening of single cells, and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0065]Example 1 A microfluidic chip for single cell capture and screening and a microfluidic chip system capable of capturing, identifying, culturing and releasing single cells

[0066] 1. Microvalve control layer and microvalve film layer

[0067] When identifying cells, light needs to pass through the microvalve control layer and the microvalve film layer. Therefore, the microvalve control layer and the microvalve film layer need to be made of transparent materials; at the same time, the microvalve control layer and the microvalve film layer need to be made of fluid The inlet and outlet ports need to be able to open holes; in addition, because the gas squeezes the film to produce bending deformation to achieve the purpose of the microvalve switch, the material of the microvalve film layer must be a soft material with good elasticity; in addition, because Cells need to be cultured on the chip, and all related materials need good biocompatibility; finally, the microvalve contr...

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Abstract

The invention provides a micro-fluidic chip applied to capture and screening of single cells, and an application thereof. The micro-fluidic chip comprises four layers of structures which are successively stacked together and mutually sealed and are respectively a micro-valve control layer, a micro-valve film layer, a substrate with a flow way and a micro-pore array and a detection release layer from top to bottom. Through introduction of a micro-processing technology and a micro-fluidic technology, the multi-layer and multi-functional micro-fluidic chip is developed, capture, identification, culture and release of cells are realized, a new method is provided for cell screening, and hyper-secretion cells can be obtained more quickly, accurately and conveniently.

Description

technical field [0001] The invention relates to a microfluidic chip for single cell capture and screening and its application. Background technique [0002] Monoclonal antibody has high specificity and high targeting, and has become an important means of tumor targeted therapy and probe detection. At present, mammalian expression systems for producing monoclonal antibodies include CHO cells, NSO cells, and hybridoma cells. During the long-term culture process, the genes expressing antibody heavy and light chains on chromosomes are deleted or rearranged, resulting in the loss of the ability of cells to secrete specific antibodies. The specific mechanism is still unclear. High-yielding cells that secrete target protein molecules tend to have lower proliferation efficiency. However, unstable heterogeneous hybridoma cells proliferate faster, and their proportion continues to expand, which affects the secretion efficiency of antibodies and becomes the key to increasing antibody ...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCC12M23/16C12M47/04
Inventor 张卫凯李仁李勤魏泽文胡志远
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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